Plant Molecular Biology 33: 947–952, 1997. 947 c 1997 Kluwer Academic Publishers. Printed in Belgium. Mini-review Narendra Tuteja International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India Received 31 December 1996; accepted in revised form 14 January 1997 Key words: DNA helicase, DNA-dependent ATPase, unwinding enzyme, DNA replication, chloroplasts Introduction since this ﬁeld is almost untouched by plant biologists. A biochemical technique to determine the unwinding Green plant cells contain three types of organelles, nuc- activity is described ﬁrst. leus, mitochondria and chloroplasts, which have their own genomes. Each genome replicates, transcribes and expresses its genetic information independent of each Biochemical assay other though there is (probably yet unknown) coordin- ation between them. DNA present in these genomes The helicase assay measures the displacement of is in the double-stranded (ds) form. However, for labelled DNA from a partial duplex molecule as many important DNA transactions, such as replica- described [19, 42]. The biochemical reaction for DNA tion, repair, recombination and in some cases transcrip- helicase catalyzed unwinding is shown in Fig. 1A. tion, the duplex DNA has to be partially unwind for Brieﬂy, the P-labelled partial duplex DNA substrate a short time
Plant Molecular Biology – Springer Journals
Published: Oct 14, 2004
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