Ultraviolet Pulsed Laser-Induced Fluorescence Nonlinearity in Optically Thick Organic Samples

Ultraviolet Pulsed Laser-Induced Fluorescence Nonlinearity in Optically Thick Organic Samples A simple two-component model is worked out to investigate pulsed laser-induced fluorescence in complex organic samples, like biological tissues, optically thick at the excitation wavelength. Expression for emitted fluorescence signal is obtained. Saturation process is shown to be determined not only by fluorophores excited by the laser, but non-fluorescent chromophores with overlapping absorption band as well. For homogeneous samples the forms of saturation curves are determined by fluorophore’s features. Experimental saturation curves of bulk paper and mice tissues ultraviolet pulsed laser-induced fluorescence are discussed considering this model. For the ns and shorter laser pulse durations with wavelength in 200–300 nm region, pulse energy density should be less than 200 μJ/cm2 for correct quantitative comparison of fluorescence spectra of biological tissues with primarily tryptophan fluorescence. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fluorescence Springer Journals

Ultraviolet Pulsed Laser-Induced Fluorescence Nonlinearity in Optically Thick Organic Samples

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Biomedicine; Biomedicine, general; Biological and Medical Physics, Biophysics; Biotechnology; Biochemistry, general; Analytical Chemistry
ISSN
1053-0509
eISSN
1573-4994
D.O.I.
10.1007/s10895-018-2232-5
Publisher site
See Article on Publisher Site

Abstract

A simple two-component model is worked out to investigate pulsed laser-induced fluorescence in complex organic samples, like biological tissues, optically thick at the excitation wavelength. Expression for emitted fluorescence signal is obtained. Saturation process is shown to be determined not only by fluorophores excited by the laser, but non-fluorescent chromophores with overlapping absorption band as well. For homogeneous samples the forms of saturation curves are determined by fluorophore’s features. Experimental saturation curves of bulk paper and mice tissues ultraviolet pulsed laser-induced fluorescence are discussed considering this model. For the ns and shorter laser pulse durations with wavelength in 200–300 nm region, pulse energy density should be less than 200 μJ/cm2 for correct quantitative comparison of fluorescence spectra of biological tissues with primarily tryptophan fluorescence.

Journal

Journal of FluorescenceSpringer Journals

Published: Apr 25, 2018

References

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