Two Kinds of Transient Outward Currents, I A and I Adepol , in F76 and D1 Soma Membranes of the Subesophageal Ganglia of Helix aspersa

Two Kinds of Transient Outward Currents, I A and I Adepol , in F76 and D1 Soma Membranes of... Transient outward currents were characterized with twin electrode voltage clamp techniques in isolated F76 and D1 neuronal membranes (soma only) of Helix aspersa subesophageal ganglia. In this study, in addition to the transient outward current (A-current, I A ) described by Connor and Stevens (1971b), another fast outward current, referred to as I Adepol here, is described for the first time. This is similar to the current component characterized in Aplysia (Furukawa, Kandel & Pfaffinger, 1992). The separation of these two current components was based on activation and steady-state inactivation curves, holding potentials and sensitivity to 4-aminopyridine (4-AP). In contrast to I A , I Adepol did not require hyperpolarizing conditioning pulses to remove inactivation; it was evoked from a holding potential of −40 mV, at which I A is completely inactivated. I Adepol shows noticeable activation at around −5 mV, whereas I A activates at around −50 mV. The time courses of I Adepol activation and inactivation were similar but slower than I A . It was found that I Adepol was more sensitive than I A to 4-AP. 4-AP at a concentration of 1 mm blocked I Adepol completely, whereas 5–6 mm 4-AP was needed to block I A completely. This current is potentially very important because it may, like other A currents, regulate firing frequency but notably, it does not require a period of hyperpolarization to be active. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Two Kinds of Transient Outward Currents, I A and I Adepol , in F76 and D1 Soma Membranes of the Subesophageal Ganglia of Helix aspersa

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 2001 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002320010038
Publisher site
See Article on Publisher Site

Abstract

Transient outward currents were characterized with twin electrode voltage clamp techniques in isolated F76 and D1 neuronal membranes (soma only) of Helix aspersa subesophageal ganglia. In this study, in addition to the transient outward current (A-current, I A ) described by Connor and Stevens (1971b), another fast outward current, referred to as I Adepol here, is described for the first time. This is similar to the current component characterized in Aplysia (Furukawa, Kandel & Pfaffinger, 1992). The separation of these two current components was based on activation and steady-state inactivation curves, holding potentials and sensitivity to 4-aminopyridine (4-AP). In contrast to I A , I Adepol did not require hyperpolarizing conditioning pulses to remove inactivation; it was evoked from a holding potential of −40 mV, at which I A is completely inactivated. I Adepol shows noticeable activation at around −5 mV, whereas I A activates at around −50 mV. The time courses of I Adepol activation and inactivation were similar but slower than I A . It was found that I Adepol was more sensitive than I A to 4-AP. 4-AP at a concentration of 1 mm blocked I Adepol completely, whereas 5–6 mm 4-AP was needed to block I A completely. This current is potentially very important because it may, like other A currents, regulate firing frequency but notably, it does not require a period of hyperpolarization to be active.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Jan 1, 2001

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