Two-Dimensional Crystalline Array Formation of Glucuronide Transporter from Escherichia coli by the Use of Polystyrene Beads for Detergent Removal

Two-Dimensional Crystalline Array Formation of Glucuronide Transporter from Escherichia coli by... n-Dodecyl-β-d-maltoside solubilized glucuronide transporter (GusB), the product of gusB gene from Escherichia coli, was treated with Bio-Beads as an agent for removing the detergent from a micellar solution under suitable combination with dimyristoylphosphatidylcholine. Optimizing conditions led to a two-dimensional crystalline array formation of GusB. The crystalline arrays appear to have a hexagonal lattice with layer group P6, the unit cell dimensions of a = b = 13.8 nm and γ = 120°. Each stain-protruding periodic unit showed approximately 11.8 ± 0.3 nm in a diameter in the inverse Fourier-filtered image to have formed with pentameric GusB (5 × 49.7 kDa). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Two-Dimensional Crystalline Array Formation of Glucuronide Transporter from Escherichia coli by the Use of Polystyrene Beads for Detergent Removal

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Publisher
Springer-Verlag
Copyright
Copyright © 2012 by Springer Science+Business Media New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-012-9521-8
Publisher site
See Article on Publisher Site

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