TRITHORAX, a Global Transcription Activator/Maintenance Protein of Drosophila melanogaster, Is Associated with the Nuclear Matrix

TRITHORAX, a Global Transcription Activator/Maintenance Protein of Drosophila melanogaster, Is... Immunofluorescence staining of sequentially extracted nuclei and Western blot analysis of proteins from the nuclear extract fractions showed for the first time that protein TRITHORAX, one of the most important proteins maintaining the tissue-specific transcriptionally active state of many Drosophila melanogaster genes, is associated with the nuclear matrix. TRITHORAX displayed similar staining at different stages of nuclear extraction and on the polytene chromosomes of the intact nuclei, as well as after partial or complete disruption of the nuclear envelope and chromosome release. This suggests that TRITHORAX bound to certain regulatory chromosome regions interacts with the adjacent elements of the nuclear scaffold, i.e., links the regions of actively transcribed genes with the nuclear matrix. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

TRITHORAX, a Global Transcription Activator/Maintenance Protein of Drosophila melanogaster, Is Associated with the Nuclear Matrix

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1022436010553
Publisher site
See Article on Publisher Site

Abstract

Immunofluorescence staining of sequentially extracted nuclei and Western blot analysis of proteins from the nuclear extract fractions showed for the first time that protein TRITHORAX, one of the most important proteins maintaining the tissue-specific transcriptionally active state of many Drosophila melanogaster genes, is associated with the nuclear matrix. TRITHORAX displayed similar staining at different stages of nuclear extraction and on the polytene chromosomes of the intact nuclei, as well as after partial or complete disruption of the nuclear envelope and chromosome release. This suggests that TRITHORAX bound to certain regulatory chromosome regions interacts with the adjacent elements of the nuclear scaffold, i.e., links the regions of actively transcribed genes with the nuclear matrix.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

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