Transposon insertion into a chromosomal copy of flhB gene is concurrent with defects in the formation of polar and lateral flagella in the bacterium Azospirillum brasilense Sp245

Transposon insertion into a chromosomal copy of flhB gene is concurrent with defects in the... Based on an example of Azospirillum brasilense Sp245, it was shown that, in bacteria with mixed flagellation, insertional mutagenesis of one of the copies of the flhB gene, which encodes a component of the flagellar protein export apparatus, may be concurrent with defects in the formation of both a constitutive polar flagellum and inducible lateral flagella. Despite the presence of a second copy of flhB in the plasmid-located gene cluster, which seems necessary for the formation of lateral flagella, the flhB1::Omegon-Km Sp245 mutant completely lost the ability to produce them. The described effect of the inactivation of flhB1 might be explained by the use of FlhBl for the assembly of both types of flagella. Since the open reading frame AZOBR_150176, which is transcribed in the same direction and codes for a hypothetical multisensor hybrid histidine kinase/response regulator, adjoins to the 3’-end of flhB1, the participation of the latter protein in the induction of the lateral flagellar synthesis in response to the increase in the density of the environment was not excluded. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Transposon insertion into a chromosomal copy of flhB gene is concurrent with defects in the formation of polar and lateral flagella in the bacterium Azospirillum brasilense Sp245

Loading next page...
 
/lp/springer_journal/transposon-insertion-into-a-chromosomal-copy-of-flhb-gene-is-W8QFRuBQMM
Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795413080061
Publisher site
See Article on Publisher Site

Abstract

Based on an example of Azospirillum brasilense Sp245, it was shown that, in bacteria with mixed flagellation, insertional mutagenesis of one of the copies of the flhB gene, which encodes a component of the flagellar protein export apparatus, may be concurrent with defects in the formation of both a constitutive polar flagellum and inducible lateral flagella. Despite the presence of a second copy of flhB in the plasmid-located gene cluster, which seems necessary for the formation of lateral flagella, the flhB1::Omegon-Km Sp245 mutant completely lost the ability to produce them. The described effect of the inactivation of flhB1 might be explained by the use of FlhBl for the assembly of both types of flagella. Since the open reading frame AZOBR_150176, which is transcribed in the same direction and codes for a hypothetical multisensor hybrid histidine kinase/response regulator, adjoins to the 3’-end of flhB1, the participation of the latter protein in the induction of the lateral flagellar synthesis in response to the increase in the density of the environment was not excluded.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Aug 14, 2013

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off