Transient and Permanent Fusion of Vesicles in Zea mays Coleoptile Protoplasts Measured in the Cell-attached Configuration

Transient and Permanent Fusion of Vesicles in Zea mays Coleoptile Protoplasts Measured in the... Exocytosis in protoplasts from Zea mays L. coleoptiles was studied using patch-clamp techniques. Fusion of individual vesicles with the plasma membrane was monitored as a step increase of the membrane capacitance (C m ). Vesicle fusion was observed as (i) An irreversible step increase in C m . (ii) Occasionally, irreversible C m steps were preceded by transient changes in C m , suggesting that the electrical connection between the vesicle with the plasma membrane opens and closes reversibly before full connection is achieved. (iii) Most frequently, however, stepwise transient changes in C m did not lead to an irreversible C m step. Within one patch of membrane capacitance steps due to transient and irreversible fusions were of similar amplitude. This suggests that the exocytosis events do not result from the fusion of vesicles with different sizes but are due to kinetically different states in a fusion process of the same vesicle type. The dwell time histogram of the transient fusion events peaked at about 100 msec. Fusion can be described with a circular three-state model for the fusion process of two fused states and one nonfused state. It predicts that energy input is required to drive the system into a prevailing direction. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Transient and Permanent Fusion of Vesicles in Zea mays Coleoptile Protoplasts Measured in the Cell-attached Configuration

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 2000 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002320001027
Publisher site
See Article on Publisher Site

Abstract

Exocytosis in protoplasts from Zea mays L. coleoptiles was studied using patch-clamp techniques. Fusion of individual vesicles with the plasma membrane was monitored as a step increase of the membrane capacitance (C m ). Vesicle fusion was observed as (i) An irreversible step increase in C m . (ii) Occasionally, irreversible C m steps were preceded by transient changes in C m , suggesting that the electrical connection between the vesicle with the plasma membrane opens and closes reversibly before full connection is achieved. (iii) Most frequently, however, stepwise transient changes in C m did not lead to an irreversible C m step. Within one patch of membrane capacitance steps due to transient and irreversible fusions were of similar amplitude. This suggests that the exocytosis events do not result from the fusion of vesicles with different sizes but are due to kinetically different states in a fusion process of the same vesicle type. The dwell time histogram of the transient fusion events peaked at about 100 msec. Fusion can be described with a circular three-state model for the fusion process of two fused states and one nonfused state. It predicts that energy input is required to drive the system into a prevailing direction.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Mar 1, 2000

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