Short Communications Incorporating Mouse Genome Mammalian Genome 12, 169–171 (2001). © Springer-Verlag New York Inc. 2001 DOI: 10.1007/s003350010238 Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection 1 2 2 2 2 Elizabeth R. Linnell, Charles P. Lerner, Kevin A. Johnson, Craig A. Leach, Thomas R. Ulrich, 2 1 William C. Rafferty, Elizabeth M. Simpson Centre for Molecular Medicine and Therapeutics, British Columbia Research Institute for Children’s & Women’s Health, Department of Medical th Genetics, University of British Columbia, 950 West 28 Avenue, Vancouver, British Columbia, V5Z 4H4, Canada The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine, 04609, USA Received: 31 May 2000 / Accepted: 8 September 2000 Transgenesis in the mouse has progressed from single gene meth- Land 1990). Implantation of injected embryos into pseudopregnant ods to multiple targeting strategies, driving the search for addi- females produced 84 pups, including 12 founders with tional drug selection markers. The puromycin resistance gene, pu- pPWL88puro, and 77 pups including 3 founders with pJ6Vpuro. romycin N-acetyltransferase (pac), which has been used success- Mice carrying the transgene were identified by Southern blot fully in mammalian cell lines, should prove valuable to mouse analysis
Mammalian Genome – Springer Journals
Published: Feb 1, 2001
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