In order to improve resistance to pathogens in chrysanthemum, we generated transgenic chrysanthemum plants heterogeneously expressing hpaG Xoo gene from Xanthomonas oryzae pv. oryzae via leaf-disc mediated Agrobacterium tumefaciens (EHA105) transformation. Polymerase chain reaction (PCR), Southern blot, and Reverse transcription PCR (RT-PCR) analysis showed that the hpaG Xoo gene was successfully integrated into the chrysanthemum genome and expressed in transgenic lines. An inoculation assay indicated that the presence of the transgene was associated with an improved level of resistance to alternaria leaf spot. The transgenic introduction of pathogen elicitor-encoding genes has a considerable potential for improving the disease resistance of ornamental plants. Furthermore, transgenic chrysanthemums flowered significantly earlier than the wild-type plants did, suggesting that the hpaG Xoo gene probably accelerated chrysanthemum development.
Russian Journal of Plant Physiology – Springer Journals
Published: Jul 7, 2010
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