Transgenic chrysanthemum plants expressing a harpinXoo gene demonstrate induced resistance to alternaria leaf spot and accelerated development

Transgenic chrysanthemum plants expressing a harpinXoo gene demonstrate induced resistance to... In order to improve resistance to pathogens in chrysanthemum, we generated transgenic chrysanthemum plants heterogeneously expressing hpaG Xoo gene from Xanthomonas oryzae pv. oryzae via leaf-disc mediated Agrobacterium tumefaciens (EHA105) transformation. Polymerase chain reaction (PCR), Southern blot, and Reverse transcription PCR (RT-PCR) analysis showed that the hpaG Xoo gene was successfully integrated into the chrysanthemum genome and expressed in transgenic lines. An inoculation assay indicated that the presence of the transgene was associated with an improved level of resistance to alternaria leaf spot. The transgenic introduction of pathogen elicitor-encoding genes has a considerable potential for improving the disease resistance of ornamental plants. Furthermore, transgenic chrysanthemums flowered significantly earlier than the wild-type plants did, suggesting that the hpaG Xoo gene probably accelerated chrysanthemum development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Transgenic chrysanthemum plants expressing a harpinXoo gene demonstrate induced resistance to alternaria leaf spot and accelerated development

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710040138
Publisher site
See Article on Publisher Site

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