Plant Mol Biol (2017) 93:171–183 DOI 10.1007/s11103-016-0554-8 Transformation of the Cyanidioschyzon merolae chloroplast genome: prospects for understanding chloroplast function in extreme environments 1 1 1 1 Maksymilian Zienkiewicz · Tomasz Krupnik · Anna Drożak · Anna Golke · Elżbieta Romanowska Received: 19 July 2016 / Accepted: 22 October 2016 / Published online: 28 October 2016 © The Author(s) 2016. This article is published with open access at Springerlink.com Abstract mutants, recombinant proteins and exogenous protein over- Key message We have successfully transformed an exthe- expression in C. merolae—a new model organism. mophilic red alga with the chloramphenicol acetyltrans- ferase gene, rendering this organism insensitive to its toxic- Keywords Stable chloroplast transformation · ity. Our work paves the way to further work with this new Cyanidioschyzon merolae · Chloramphenicol modelorganism. acetyltransferase · PEG · Biolistic bombardment Abstract Here we report the first successful attempt to achieve a stable, under selectable pressure, chloroplast transformation in Cyanidioschizon merolae—an extremo- Introduction philic red alga of increasing importance as a new model organism. The following protocol takes advantage of a Cyanidioschyzon merolae is an extremophilic red micro- double homologous recombination phenomenon in the alga that dwells in moderately high temperatures (40– chloroplast, allowing to introduce an exogenous, selectable 56 °C) and highly acidic (between pH 0.2–4)
Plant Molecular Biology – Springer Journals
Published: Oct 28, 2016
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