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- Publisher
- Springer Journals
- Copyright
- Copyright © 2002 by MAIK “Nauka/Interperiodica”
- Subject
- Biomedicine; Human Genetics
- ISSN
- 1022-7954
- eISSN
- 1608-3369
- DOI
- 10.1023/A:1020279429009
- Publisher site
- See Article on Publisher Site
Abstract
To transform Chlamydomonas reinhardtiiDang. cells, plasmid pCTVHyg was constructed with the use of theEscherichia colihygromycin phosphotransferase gene (hpt) controlled by the SV40 early promoter. Cells of the CW-15 mutant strain were transformed by electroporation, with the yield reaching 103 hygromycin-resistant (HygR) clones per 106 recipient cells. The exogenous DNA integrated in the Ch. reinhardtii nuclear genome showed stable transmission for approximately 350 cell generations, while hygromycin resistance was expressed as an unstable character. Codon usage was compared for the hptgene and Ch. reinhardtiinuclear genes. The results testified that codon usage bias, which is characteristic of Ch. reinhardtii, is not the major factor affecting foreign gene expression. The advantages of the selective system for studying Ch. reinhardtii transformation with heterologous genes are discussed.
Journal
Russian Journal of Genetics – Springer Journals
Published: Oct 13, 2004