Transfer of genetic constructions through the transplacental barrier into mice embryos

Transfer of genetic constructions through the transplacental barrier into mice embryos Genetic modification of mammalian embryos is an important way to model various changes in human development; also, it is an instrument for studying the functions of certain genes in mammals. Using our own experience in developing modes of delivery of genetic constructions to mammals in a nonviral way, we present here data on the delivery of a eukaryotic expression vector to mice embryos through the transpla-cental barrier with the use of hydrodynamic intravenous injections of DNA-hybrid peptide complexes to pregnant females. The peptide has a cationic part for interaction with DNA and includes a ligand structure towards receptors of the releasing factor of luteinizing hormone (RFLH, luliberin). Advantages of the suggested method are simplicity, economy, nonimmunogenicity for females, and the ability to multiply repeat the procedure. On the basis of the method, systemic gene delivery into tissues of mammalian embryos may be developed. Russian Journal of Developmental Biology Springer Journals

Transfer of genetic constructions through the transplacental barrier into mice embryos

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SP MAIK Nauka/Interperiodica
Copyright © 2010 by Pleiades Publishing, Ltd.
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
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