Transcriptome analysis reveals differentially expressed genes (DEGs) related to lettuce (Lactuca sativa) treated by TiO2/ZnO nanoparticles

Transcriptome analysis reveals differentially expressed genes (DEGs) related to lettuce (Lactuca... The widespread use of nanomaterials raises concern over their effects and roles in plants. Many researches have proved that nanoparticles could promote the growth and development of plants, so as our previous experiments in lettuce. However, the molecular regulation mechanisms involved in the lettuce treated by nanoparticles remain largely unknown. Herein, a cDNA library of lettuce (Lactuca sativa) leaf and root in the middle growth stage (25th day after transplanting) was constructed. Approximately 10.4 GB clean reads were obtained, and a total of 36919 unigenes were formed by initial sequence splicing, with an average read length of 897 bp. 29136 unigenes (78.92%) were annotated based on at least one database. 509 genes were identified as differentially expressed genes (DEGs) in leaf treated by nanomateials, which comprised 223 up-regulated genes and 286 down-regulated genes, while 3666 genes were identified as DEGs in root, which comprised 844 up-regulated genes and 2822 down-regulated genes. Hundreds of genes potentially involved in nanoparticles were identified from these DEGs, including the genes related to photosynthetic metabolism, antioxidant enzymes, nitrogen metabolism and sucrose and starch metabolic pathways. Ultimately, a preliminary regulatory mechanism of growth and development of lettuce treated by TiO2/ZnO nanoparticles was proposed based on transcriptome analyses and published documents. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Growth Regulation Springer Journals

Transcriptome analysis reveals differentially expressed genes (DEGs) related to lettuce (Lactuca sativa) treated by TiO2/ZnO nanoparticles

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Publisher
Springer Netherlands
Copyright
Copyright © 2017 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Plant Anatomy/Development; Plant Physiology; Agriculture
ISSN
0167-6903
eISSN
1573-5087
D.O.I.
10.1007/s10725-017-0280-5
Publisher site
See Article on Publisher Site

Abstract

The widespread use of nanomaterials raises concern over their effects and roles in plants. Many researches have proved that nanoparticles could promote the growth and development of plants, so as our previous experiments in lettuce. However, the molecular regulation mechanisms involved in the lettuce treated by nanoparticles remain largely unknown. Herein, a cDNA library of lettuce (Lactuca sativa) leaf and root in the middle growth stage (25th day after transplanting) was constructed. Approximately 10.4 GB clean reads were obtained, and a total of 36919 unigenes were formed by initial sequence splicing, with an average read length of 897 bp. 29136 unigenes (78.92%) were annotated based on at least one database. 509 genes were identified as differentially expressed genes (DEGs) in leaf treated by nanomateials, which comprised 223 up-regulated genes and 286 down-regulated genes, while 3666 genes were identified as DEGs in root, which comprised 844 up-regulated genes and 2822 down-regulated genes. Hundreds of genes potentially involved in nanoparticles were identified from these DEGs, including the genes related to photosynthetic metabolism, antioxidant enzymes, nitrogen metabolism and sucrose and starch metabolic pathways. Ultimately, a preliminary regulatory mechanism of growth and development of lettuce treated by TiO2/ZnO nanoparticles was proposed based on transcriptome analyses and published documents.

Journal

Plant Growth RegulationSpringer Journals

Published: May 24, 2017

References

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