Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing

Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet... Finger millet (Eleusine coracana L.) is a hardy cereal known for its superior level of tolerance against drought, salinity, diseases and its nutritional properties. In this study, attempts were made to unravel the physiological and molecular basis of salinity tolerance in two contrasting finger millet genotypes viz., CO 12 and Trichy 1. Physiological studies revealed that the tolerant genotype Trichy 1 had lower Na+ to K+ ratio in leaves and shoots, higher growth rate (osmotic tolerance) and ability to accumulate higher amount of total soluble sugar in leaves under salinity stress. We sequenced the salinity responsive leaf transcriptome of contrasting finger millet genotypes using IonProton platform and generated 27.91 million reads. Mapping and annotation of finger millet transcripts against rice gene models led to the identification of salinity responsive genes and genotype specific responses. Several functional groups of genes like transporters, transcription factors, genes involved in cell signaling, osmotic homeostasis and biosynthesis of compatible solutes were found to be highly up-regulated in the tolerant Trichy 1. Salinity stress inhibited photosynthetic capacity and photosynthesis related genes in the susceptible genotype CO 12. Several genes involved in cell growth and differentiation were found to be up-regulated in both the genotypes but more specifically in tolerant genotype. Genes involved in flavonoid biosynthesis were found to be down-regulated specifically in the salinity tolerant Trichy 1. This study provides a genome-wide transcriptional analysis of two finger millet genotypes differing in their level of salinity tolerance during a gradually progressing salinity stress under greenhouse conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing

Loading next page...
 
/lp/springer_journal/transcriptome-analysis-of-salinity-responsiveness-in-contrasting-lfA6cQJpH0
Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-014-0199-4
Publisher site
See Article on Publisher Site

Abstract

Finger millet (Eleusine coracana L.) is a hardy cereal known for its superior level of tolerance against drought, salinity, diseases and its nutritional properties. In this study, attempts were made to unravel the physiological and molecular basis of salinity tolerance in two contrasting finger millet genotypes viz., CO 12 and Trichy 1. Physiological studies revealed that the tolerant genotype Trichy 1 had lower Na+ to K+ ratio in leaves and shoots, higher growth rate (osmotic tolerance) and ability to accumulate higher amount of total soluble sugar in leaves under salinity stress. We sequenced the salinity responsive leaf transcriptome of contrasting finger millet genotypes using IonProton platform and generated 27.91 million reads. Mapping and annotation of finger millet transcripts against rice gene models led to the identification of salinity responsive genes and genotype specific responses. Several functional groups of genes like transporters, transcription factors, genes involved in cell signaling, osmotic homeostasis and biosynthesis of compatible solutes were found to be highly up-regulated in the tolerant Trichy 1. Salinity stress inhibited photosynthetic capacity and photosynthesis related genes in the susceptible genotype CO 12. Several genes involved in cell growth and differentiation were found to be up-regulated in both the genotypes but more specifically in tolerant genotype. Genes involved in flavonoid biosynthesis were found to be down-regulated specifically in the salinity tolerant Trichy 1. This study provides a genome-wide transcriptional analysis of two finger millet genotypes differing in their level of salinity tolerance during a gradually progressing salinity stress under greenhouse conditions.

Journal

Plant Molecular BiologySpringer Journals

Published: May 18, 2014

References

  • Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp
    Allakhverdiev, SI; Sakamoto, A; Nishiyama, Y; Inaba, M; Murata, N
  • Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.)
    Anuradha, S; Rao, SSR
  • Potential biochemical indicators of salinity tolerance in plants
    Ashraf, M; Harris, P
  • Alfin1, a novel zinc-finger protein in alfalfa roots that binds to promoter elements in the salt-inducible MsPRP2 gene
    Bastola, DR; Pethe, VV; Winicov, I
  • Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings
    Borsani, O; Valpuesta, V; Botella, MA
  • Molecular responses to water deficit
    Bray, EA
  • Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana
    Brini, F; Hanin, M; Lumbreras, V; Amara, I; Khoudi, H; Hassairi, A; Pagès, M; Masmoudi, K
  • Effects of NaCl salinity on growth, ion content and CO2 assimilation rate of six olive cultivars
    Chartzoulakis, K; Loupassaki, M; Bertaki, M; Androulakis, I
  • BRASSINOSTEROIDS: essential Regulators of Plant Growth and Development
    Clouse, SD; Sasse, JM
  • Control of sodium transport in durum wheat
    Davenport, R; James, RA; Zakrisson-Plogander, A; Tester, M; Munns, R
  • Proline biosynthesis and osmoregulation in plants
    Delauney, AJ; Verma, DPS
  • Comparative genetics in the grasses
    Devos, KM; Gale, MD
  • The maize abscisic acid-responsive protein Rab17 is located in the nucleus and interacts with nuclear localization signals
    Goday, A; Jensen, AB; Culianez-Macia, FA; Albà, MM; Figueras, M; Serratosa, J; Torrent, M; Pagès, M
  • Jasmonic acid differentially affects growth, ion uptake and abscisic acid concentration in salt-tolerant and salt-sensitive rice cultivars
    Kang, D; Seo, Y; Lee, J; Ishii, R; Kim, K; Shin, D; Park, S; Jang, S; Lee, I
  • Gene expression profiles during the initial phase of salt stress in rice
    Kawasaki, S; Borchert, C; Deyholos, M; Wang, H; Brazille, S; Kawai, K; Galbraith, D; Bohnert, HJ
  • Overexpression of [delta]-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants
    Kishor, PK; Hong, Z; Miao, GH; Hu, CAA; Verma, DPS
  • The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity
    Koag, MC; Fenton, RD; Wilkens, S; Close, TJ
  • Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress
    Kreps, JA; Wu, Y; Chang, HS; Zhu, T; Wang, X; Harper, JF
  • Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L
    Kumari, S; nee Sabharwal, VP; Kushwaha, HR; Sopory, SK; Singla Pareek, SL; Pareek, A

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off