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E. Brown (1983)
Chloroplasts and mitochondriaPhytochemistry, 22
S. Newman, J. Boynton, N. Gillham, Barbara Randolph-Anderson, Anita Johnson, E. Harris (1990)
Transformation of chloroplast ribosomal RNA genes in Chlamydomonas: molecular and genetic characterization of integration events.Genetics, 126 4
(1966)
Cytochrome f and plastocyanin: their sequence in the photoelectric transport chain
M. Goldschmidt-Clermont, M. Rahire (1986)
Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii.Journal of molecular biology, 191 3
Chi-Lien Cheng, G. Acedo, Michael Cristinsin, M. Conkling (1992)
Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription.Proceedings of the National Academy of Sciences of the United States of America, 89
(1982)
Regulation of the nitrate-reducing system enzymes in wild and mutant strains from Chlamydomonas reinhardtii. MolGen. Genet, 186
S. Pouteau, I. Chérel, H. Vaucheret, M. Caboche (1989)
Nitrate Reductase mRNA Regulation in Nicotiana plumbaginifolia Nitrate Reductase-Deficient Mutants.The Plant cell, 1
T. Hoff, H. Truong, M. Caboche (1994)
The use of mutants and transgenic plants to study nitrate assimilationPlant Cell and Environment, 17
M. Guerrero, A. Vega, M. Losada (1981)
The Assimilatory Nitrate-Reducing System and its RegulationAnnual Review of Plant Biology, 32
H. Vaucheret, A. Marion-Poll, C. Meyer, J. Faure, E. Marín, M. Caboche (1992)
Interest in and limits to the utilization of reporter genes for the analysis of transcriptional regulation of nitrate reductaseMolecular and General Genetics MGG, 235
C. Hipkin, B. Al-Bassam, P. Syrett (1980)
The roles of nitrate and ammonium in the regulation of the development of nitrate reductase in Chlamydomonas reinhardiiPlanta, 150
E. Fernández, R. Matagne (2004)
In vivo complementation analysis of nitrate reductase-deficient mutants in Chlamydomonas reinhardtiiCurrent Genetics, 10
E. Fernández, R. Schnell, L. Ranum, Susanne Hussey, C. Silflow, P. Lefebvre (1989)
Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America, 86 17
C. Silflow, J. Rosenbaum (1981)
Multiple α- and β-tubulin genes in chlamydomonas and regulation of tubulin mRNA levels after deflagellationCell, 24
A. Franco, J. Cárdenas, E. Fernández (1987)
Involvement of Reversible Inactivation in the Regulation of Nitrate Reductase Enzyme Levels in Chlamydomonas reinhardtii.Plant physiology, 84 3
T. Lien, O. Schreiner, M. Steine (1975)
Purification of a derepressible arylsulfatase from Chlamydomonas reinhardti. Properties of the enzyme in intact cells and in purified state.Biochimica et biophysica acta, 384 1
E. Fernández, J. Cárdenas (1989)
Genetics and regulatory aspects of nitrate assimilation in algae.
K. Kalakoutskii, E. Fernández (1995)
Chlamydomonas reinhardtii nitrate reductase complex has 105 kDa subunits in the wild-type strain and a structural mutantPlant Science, 105
E. Harris (1989)
The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use
N. Dunn-Coleman, J. Smarrelli, R. Garrett (1984)
Nitrate Assimilation in Eukaryotic CellsInternational Review of Cytology-a Survey of Cell Biology, 92
(1996)
Properties of the enzyme in intact cells and in a purified state
John Wray, Philip Filner (1970)
Structural and functional relationships of enzyme activities induced by nitrate in barley.The Biochemical journal, 119 4
M. Dionisio-Sese, H. Fukuzawa, S. Miyachi (1990)
Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii.Plant physiology, 94 3
R. Hageman, A. Reed (1980)
[24] Nitrate reductase from higher plantsMethods in Enzymology, 69
K. Steinback, L. Mcintosh, L. Bogorad, C. Arntzen (1981)
Identification of the triazine receptor protein as a chloroplast gene product.Proceedings of the National Academy of Sciences of the United States of America, 78 12
(1950)
Some approaches to the study of the role of metals in the metabolism of microorganismsProc. Am. Phil. Soc., 94
A. Quesada, I. Gómez, E. Fernández (1998)
Clustering of the nitrite reductase gene and a light-regulated gene with nitrate assimilation loci in Chlamydomonas reinhardtiiPlanta, 206
Rafael Prieto, Alain Dubus, A. Galván, E. Fernández (1996)
Isolation and characterization of two new negative regulatory mutants for nitrate assimilation inChlamydomonas reinhardtii obtained by insertional mutagenesisMolecular and General Genetics MGG, 251
M. Deng, T. Moureaux, I. Chérel, J. Boutin, M. Caboche (1991)
Effects of nitrogen metabolites on the regulation and circadian expression of tobacco nitrate reductasePlant Physiology and Biochemistry, 29
J. Vega, J. Herrera, P. Aparicio, A. Paneque, M. Losada (1971)
Role of molybdenum in nitrate reduction by chlorella.Plant physiology, 48 3
N. Shiraishi, Takahide Sato, N. Ogura, H. Nakagawa (1992)
Control by glutamine of the synthesis of nitrate reductase in cultured spinach cellsPlant and Cell Physiology, 33
M. Adam, R. Loppes (1998)
Use of the ARG7 gene as an insertional mutagen to clone PHON 24, a gene required for derepressible neutral phosphatase activity in Chlamydomonas reinhardtiiMolecular and General Genetics MGG, 258
R. Schnell, P. Lefebvre (1993)
Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging.Genetics, 134 3
A. Franco, J. Cárdenas, E. Fernández (1988)
Regulation by ammonium of nitrate and nitrite assimilation in Chlamydomonas reinhardtii.Biochimica et biophysica acta, 951 1
E. Fernández, A. Galván, A. Quesada (1998)
Nitrogen Assimilation and its Regulation
(1992)
Expression of the arylsulfatase gene from the β2-tubulin promoter in Chlamydomonas reinhardtii. Nucl.Acids Res., 20
M. Deng, T. Moureaux, M. Caboche (1989)
Tungstate, a molybdate analog inactivating nitrate reductase, deregulates the expression of the nitrate reductase structural gene.Plant physiology, 91 1
E. Hostos, J. Schilling, A. Grossman (1989)
Structure and expression of the gene encoding the periplasmic arylsulfatase of Chlamydomonas reinhardtiiMolecular and General Genetics MGG, 218
J. Davies, D. Weeks, A. Grossman (1992)
Expression of the arylsulfatase gene from the beta 2-tubulin promoter in Chlamydomonas reinhardtii.Nucleic acids research, 20 12
(1981)
The assimila-tory nitrate-reducing system and its regulationAnnu. Rev. Plant Physiol., 32
K. Kindle (1987)
Expression of a gene for a light-harvesting chlorophyll a/b-binding protein in Chlamydomonas reinhardtii: effect of light and acetatePlant Molecular Biology, 9
A. Quesada, A. Galván, R. Schnell, P. Lefebvre, E. Fernández (1993)
Five nitrate assimilation-related loci are clustered in Chlamydomonas reinhardtiiMolecular and General Genetics MGG, 240
Donghong Zhang, P. Lefebvre (1997)
FAR1, a negative regulatory locus required for the repression of the nitrate reductase gene in Chlamydomonas reinhardtii.Genetics, 146 1
D. Davies, A. Plaskitt (1971)
Genetical and structural analyses of cell-wall formation in Chlamydomonas reinhardiGenetics Research, 17
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
A. Quesada, E. Fernández (2004)
Expression of nitrate assimilation related genes in Chlamydomonas reinhardtiiPlant Molecular Biology, 24
M. Vincentz, T. Moureaux, M. Leydecker, H. Vaucheret, M. Caboche (1993)
Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites.The Plant journal : for cell and molecular biology, 3 2
唐丹玲, 齐雨藻, 罗宇华 (1993)
莱哈衣藻（Chlamydomonas reinhardtii ..., 14
Qiang Chen, C. Silflow (1996)
Isolation and Characterization of Glutamine Synthetase Genes in Chlamydomonas reinhardtii, 112
B. Notton, E. Hewitt (1971)
The role of tungsten in the inhibition of nitrate reductase activity in spinach (spinacea oleracea L.) leaves.Biochemical and biophysical research communications, 44 3
M. Ares, S. Howell (1982)
Cell cycle stage-specific accumulation of mRNAs encoding tubulin and other polypeptides in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America, 79 18
A. Galván, J. Cárdenas, E. Fernández (1992)
Nitrate Reductase Regulates Expression of Nitrite Uptake and Nitrite Reductase Activities in Chlamydomonas reinhardtii.Plant physiology, 98 2
E. Hostos, R. Togasaki, A. Grossman (1988)
Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtiiThe Journal of Cell Biology, 106
M. Navarro, R. Prieto, E. Fernández, A. Galván (1996)
Constitutive expression of nitrate reductase changes the regulation of nitrate and nitrite transporters in Chlamydomonas reinhardtiiPlant Journal, 9
J. Callaci, J. Smarrelli (1991)
Regulation of the inducible nitrate reductase isoform from soybeans.Biochimica et biophysica acta, 1088 1
M. Ohresser, R. Matagne, R. Loppes (1997)
Expression of the arylsulphatase reporter gene under the control of the nit1 promoter in Chlamydomonas reinhardtiiCurrent Genetics, 31
N. Crawford, W. Campbell, R. Davis (1986)
Nitrate reductase from squash: cDNA cloning and nitrate regulation.Proceedings of the National Academy of Sciences of the United States of America, 83 21
The NAD(P)H nitrate reductase (NR) from Chlamydomonas reinhardtii is encoded by the structural gene Nia1. Numerous data from the literature indicate that this enzyme is submitted to complex regulation mechanisms involving multiple controls at transcriptional and post-transcriptional levels. To specifically investigate the regulation of the Nia1 gene at the transcriptional level, NR+ and NR− transformed cells harbouring the Nia1:Ars construct (Nia1 promoter fused to the arylsulfatase (ARS)-encoding Ars reporter gene) were cultivated under various experimental conditions and the ARS activities were recorded. ARS levels were very low in cells grown in the presence of NH4Cl and dramatically increased on agar medium deprived of any nitrogen source or containing nitrate, nitrite, urea, arginine or glutamine. Compared to nitrogen-free medium, a slight positive effect of nitrate in the NR+ strain and a significant negative effect of nitrite in both NR+ and NR− strains were observed. The ARS activities were high in the light and very low in the dark or in the light in the presence of DCMU, indicating that Nia1 transcription is strikingly dependent on photosynthetic activity. Acetate used as a carbon source in the dark did not substitute for light in stimulating Nia1:Ars expression. Inactivation of NR by tungstate treatment of the NR+ strain resulted in a dramatic increase of ARS level suggesting that in Chlamydomonas, like in higher plants, active NR negatively regulates the transcription of the NR structural gene. Deleting the major part of the Nia1 leader sequence still present in the chimeric gene resulted in a decrease of ARS level but did not modify the regulation pattern.
Plant Molecular Biology – Springer Journals
Published: Sep 28, 2004
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