Transcriptional Regulation of ABI3- and ABA-responsive Genes Including RD29B and RD29A in Seeds, Germinating Embryos, and Seedlings of Arabidopsis

Transcriptional Regulation of ABI3- and ABA-responsive Genes Including RD29B and RD29A in Seeds,... ABA-responsive elements (ABREs) are cis-acting elements and basic leucine zipper (bZIP)-type ABRE-binding proteins (AREBs) are transcriptional activators that function in the expression of RD29B in vegetative tissue of Arabidopsis in response to abscisic acid (ABA) treatment. Dehydration-responsive elements (DREs) function as coupling elements of ABRE in the expression of RD29A in response to ABA. Expression analysis using abi3 and abi5 mutants showed that ABI3 and ABI5 play important roles in the expression of RD29B in seeds. Base-substitution analysis showed that two ABREs function strongly and one ABRE coupled with DRE functions weakly in the expression of RD29A in embryos. In a transient transactivation experiment, ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly but these proteins activated the transcription driven by the RD29A promoter weakly. In 35S::ABI3 Arabidopsis plants, the expression of RD29B was up-regulated strongly, but that of RD29A was up-regulated weakly. These results indicate that the expression of RD29B having ABREs in the promoter is up-regulated strongly by ABI3, whereas that of RD29A having one ABRE coupled with DREs in the promoter is up-regulated weakly by ABI3. We compared the expression of 7000 Arabidopsis genes in response to ABA treatment during germination and in the vegetative growth stage, and that in 35S::ABI3 plants using a full-length cDNA microarray. The expression of ABI3- and/or ABA-responsive genes and cis-elements in the promoters are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Transcriptional Regulation of ABI3- and ABA-responsive Genes Including RD29B and RD29A in Seeds, Germinating Embryos, and Seedlings of Arabidopsis

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2006 by Springer
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-005-2418-5
Publisher site
See Article on Publisher Site

Abstract

ABA-responsive elements (ABREs) are cis-acting elements and basic leucine zipper (bZIP)-type ABRE-binding proteins (AREBs) are transcriptional activators that function in the expression of RD29B in vegetative tissue of Arabidopsis in response to abscisic acid (ABA) treatment. Dehydration-responsive elements (DREs) function as coupling elements of ABRE in the expression of RD29A in response to ABA. Expression analysis using abi3 and abi5 mutants showed that ABI3 and ABI5 play important roles in the expression of RD29B in seeds. Base-substitution analysis showed that two ABREs function strongly and one ABRE coupled with DRE functions weakly in the expression of RD29A in embryos. In a transient transactivation experiment, ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly but these proteins activated the transcription driven by the RD29A promoter weakly. In 35S::ABI3 Arabidopsis plants, the expression of RD29B was up-regulated strongly, but that of RD29A was up-regulated weakly. These results indicate that the expression of RD29B having ABREs in the promoter is up-regulated strongly by ABI3, whereas that of RD29A having one ABRE coupled with DREs in the promoter is up-regulated weakly by ABI3. We compared the expression of 7000 Arabidopsis genes in response to ABA treatment during germination and in the vegetative growth stage, and that in 35S::ABI3 plants using a full-length cDNA microarray. The expression of ABI3- and/or ABA-responsive genes and cis-elements in the promoters are discussed.

Journal

Plant Molecular Biology – Springer Journals

Published: Aug 24, 2005

References

  • Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling
    Abe, H.; Urao, T.; Ito, T.; Seki, M.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis
    Bensmihen, S.; Rippa, S.; Lambert, G.; Jublot, D.; Pautot, V.; Granier, F.; Giraudat, J.; Parcy, F.
  • Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response
    Brocard, I.M.; Lynch, T. J.; Finkelstein, R.R.
  • Regulatory networks in seeds integrating developmental, abscisic acid, sugar, and light signaling
    Brocard-Gifford, I.M.; Lynch, T.J.; Finkelstein, R.R.
  • Regulation of Arabidopsis thaliana Em genes: role of ABI5
    Carles, C.; Bies-Etheve, N.; Aspart, L.; Léon-Kloosterziel, K.M.; Koornneef, M.; Echeverria, M.; Delseny, M.
  • Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants
    Chinnusamy, V.; Schumaker, K.; Zhu, J.-K.
  • ABFs, a family of ABA-responsive element binding factors
    Choi, H.; Hong, J.; Ha, J.; Kang, J.; Kim, S.Y.
  • Abscisic acid signaling in seeds and seedlings
    Finkelstein, R.R.; Gampala, S.S.L.; Rock, C.D.
  • The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor
    Finkelstein, R.; Lynch, T.
  • A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway
    Fujita, M.; Fujita, Y.; Maruyama, K.; Seki, M.; Hiratsu, K.; Ohme-Takagi, M.; Tran, L.-S.P.; Yamaguchi-Shinozaki, K.; Shinozaki, K.
  • Mutational analysis of the tobacco mosaic virus 5′-leader for altered ability to enhance translation
    Gallie, D.R.; Sleat, D.E.; Watts, J.W.; Turner, P.C.; Wilson, T.M.
  • Isolation of the Arabidopsis ABI3 gene by positional cloning
    Giraudat, J.; Hauge, B.M.; Valon, C.; Smalle, J.; Parcy, F.; Goodman, H.M.
  • Experimentally determined sequence requirement of ACGT-containing abscisic acid response element
    Hattori, T.; Totsuka, M.; Hobo, T.; Kagaya, Y.; Yamamoto-Toyoda, A.
  • The Viviparous-1 gene and abscisic acid activate the C1 regulatory gene for anthocyanin biosynthesis during seed maturation in maize
    Hattori, T.; Vasil, V.; Rosenkrans, L.; Hannah, L.C.; McCarty, D.R.; Vasil, I.K.
  • ACGT-containing abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent
    Hobo, T.; Asada, M.; Kowyama, Y.; Hattori, T.
  • bZIP transcription factors in Arabidopsis
    Jakoby, M.; Weisshaar, B.; Droge-Laser, W.; Vicente-Carbajosa, J.; Tiedemann, J.; Kroj, T.; Parcy, F.
  • Indirect ABA-dependent regulation of seed storage protein genes by FUSCA3 transcription factor in Arabidopsis
    Kagaya, Y.; Okuda, R.; Ban, A.; Toyoshima, R.; Tsutsumida, K.; Usui, H.; Yamamoto, A.; Hattori, T.
  • Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling
    Kang, J.; Choi, H.; Im, M.; Kim, S.Y.
  • Localization and interaction of the cis-acting elements for abscisic acid, VIVIPAROUS1, and light activation of the C1 gene of maize
    Kao, C.-Y.; Cocciolone, S.M.; Vasil, I.K.; McCarty, D.R.
  • Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis
    Liu, Q.; Kasuga, M.; Sakuma, Y.; Abe, H.; Miura, S.; Yamaguchi-Shinozaki, K.; Shinozaki, K.
  • A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana
    Lopez-Molina, L.; Chua, N.-H.
  • A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis
    Lopez-Molina, L.; Mongrand, S.; Chua, N.-H.
  • ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination
    Lopez-Molina, L.; Mongrand, S.; McLachlin, D.T.; Chait, B.T.; Chua, N.-H.
  • Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems
    Maruyama, K.; Sakuma, Y.; Kasuga, M.; Ito, Y.; Seki, M.; Goda, H.; Shimada, Y.; Yoshida, S.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • The Viviparous-1developmental gene of maize encodes a novel transcriptional activator
    McCarty, D.R.; Hattori, T.; Carson, C.B.; Vasil, V.; Lazar, M.; Vasil, I.K.
  • Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants
    Mitsuhara, I.; Ugaki, M.; Hirochika, H.; Ohshima, M.; Murakami, T.; Gotoh, Y.; Katayose, Y.; Nakamura, S.; Honkura, R.; Nishimiya, S.; Ueno, K.; Mochizuki, A.; Tanimoto, H.; Tsugawa, H.; Otsuki, Y.; Ohashi, Y.
  • Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA
    Monke, G.; Altschmied, L.; Tewes, A.; Reidt, W.; Mock, H.-P.; Baumlein, H.; Conrad, U.
  • Physical interactions between ABA response loci of Arabidopsis
    Nakamura, S.; Lynch, T.J.; Finkelstein, R.R.
  • Molecular Methods of Plant Analysis, Vol.22: Testing for Genetic Manipulation in Plants
    Nakashima, K.; Yamaguchi-Shinozaki, K.
  • Molecular studies on stress-responsive gene expression in Arabidopsis and improvement of stress tolerance in crop plants by regulon biotechnology
    Nakashima, K.; Yamaguchi-Shinozaki, K.
  • Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene
    Nambara, E.; Keith, K.; McCourt, P.; Naito, S.
  • Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses
    Narusaka, Y.; Nakashima, K.; Shinwari, Z.K.; Sakuma, Y.; Furihata, T.; Abe, H.; Narusaka, M.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid
    Parcy, F.; Valon, C.; Raynal, M.; Gaubier-Comella, P.; Delseny, M.; Giraudat, J.
  • DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression
    Sakuma, Y.; Liu, Q.; Dubouzet, J.G.; Abe, H.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis
    Satoh, R.; Fujita, Y.; Nakashima, K.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray
    Seki, M.; Ishida, J.; Narusaka, M.; Fujita, M.; Nanjo, T.; Umezawa, T.; Kamiya, A.; Nakajima, M.; Enju, A.; Sakurai, T.; Satou, M.; Akiyama, K.; Yamaguchi-Shinozaki, K.; Carninci, P.; Kawai, J.; Hayashizaki, Y.; Shinozaki, K.
  • Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray
    Seki, M.; Narusaka, M.; Ishida, J.; Nanjo, T.; Fujita, M.; Oono, Y.; Kamiya, A.; Nakajima, M.; Enju, A.; Sakurai, T.; Satou, M.; Akiyama, K.; Taji, T.; Yamaguchi-Shinozaki, K.; Carninci, P.; Kawai, J.; Hayashizaki, Y.; Shinozaki, K.
  • Functional annotation of a full-length Arabidopsis cDNA collection
    Seki, M.; Narusaka, M.; Kamiya, A.; Ishida, J.; Satou, M.; Sakurai, T.; Nakajima, M.; Enju, A.; Akiyama, K.; Oono, Y.; Muramatsu, M.; Hayashizaki, Y.; Kawai, J.; Carninci, P.; Itoh, M.; Ishii, Y.; Arakawa, T.; Shibata, K.; Shinagawa, A.; Shinozaki, K.
  • Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABA-responsive complexes each containing a G-box and a novel cis-acting element
    Shen, Q.; Ho, T.-H.D.
  • Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley
    Shen, Q.; Zhang, P.; Ho, T.-H.D.
  • Functional definition of ABA-response complexes: the promoter units necessary and sufficient for ABA induction of gene expression in barley (Hordeum vulgare L.)
    Shen, Q.J.; Casaretto, J.A.; Zhang, P.; Ho, T.-H.D.
  • Regulatory network of gene expression in the drought and cold stress responses
    Shinozaki, K.; Yamaguchi-Shinozaki, K.; Seki, M.
  • Viviparous1 alters global gene expression patterns through regulation of abscisic acid signaling
    Suzuki, M.; Ketterling, M.G.; Li, Q.-B.; McCarty, D.R.
  • Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter
    Tran, L.-S.P.; Nakashima, K.; Sakuma, Y.; Simpson, S.D.; Fujita, Y.; Maruyama, K.; Fujita, M.; Seki, M.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions
    Uno, Y.; Furihata, T.; Abe, H.; Yoshida, R.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
  • Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants
    Yamaguchi-Shinozaki, K.; Shinozaki, K.
  • A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress
    Yamaguchi-Shinozaki, K.; Shinozaki, K.
  • Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters
    Yamaguchi-Shinozaki, K.; Shinozaki, K.

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