Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region of the bovine α S1 casein gene in the mammary gland of transgenic mice and in mouse embryonic stem cells

Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region... The regulatory region of the bovine αS1 casein gene was used to obtain two genetic constructs for expression of human lactoferrin in the mammary gland of transgenic animals. Several transfected mouse embryonic stem cell (ESC) lines and primary transgenic mice were generated with these constructs. Recombinant lactoferrin was not detected in milk of transgenic mice by Western blotting. However, a recombinant transcript was found in RNAs isolated from mammary glands of transgenic females during lactation and from transfected ESC lines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region of the bovine α S1 casein gene in the mammary gland of transgenic mice and in mouse embryonic stem cells

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2005 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1007/s11177-005-0077-x
Publisher site
See Article on Publisher Site

Abstract

The regulatory region of the bovine αS1 casein gene was used to obtain two genetic constructs for expression of human lactoferrin in the mammary gland of transgenic animals. Several transfected mouse embryonic stem cell (ESC) lines and primary transgenic mice were generated with these constructs. Recombinant lactoferrin was not detected in milk of transgenic mice by Western blotting. However, a recombinant transcript was found in RNAs isolated from mammary glands of transgenic females during lactation and from transfected ESC lines.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Apr 12, 2005

References

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