Transcript accumulation and utilization of alternate and non-consensus splice sites in rice granule-bound starch synthase are temperature-sensitive and controlled by a single-nucleotide polymorphism

Transcript accumulation and utilization of alternate and non-consensus splice sites in rice... Granule-bound starch synthase (GBSS), a product of the waxy gene in rice (Oryza sativa L.), is necessary for the synthesis of amylose in the endosperm. In an extended pedigree of 89 rice cultivars, we have previously shown that all cultivars with more than 18% amylose had the sequence AGGTATA at the leader intron 5′ splice site, while all cultivars with a lower proportion of amylose had the sequence AGTTATA. This single-nucleotide polymorphism reduces the efficiency of GBSS pre-mRNA processing. It also results in alternate splicing at multiple sites, some of which have non-consensus sequences. Here we demonstrate that this same G-to-T polymorphism is also associated with differential sensitivity to temperature during the period of grain development. Cultivars with the sequence AGTTATA have a substantial increase in accumulation of mature GBSS transcripts at 18 °C compared to 25 or 32 °C. The selection of leader intron 5′ splice sites is also affected by temperature in these cultivars. A 5′ splice site −93 upstream from that used in high-amylose varieties predominates at 18 °C. At higher temperatures there is increased utilization of a 5′ splice site at −1 and a non-consensus site at +1. Potential implications of differential 5′ splice site selection and associated differences in 3′ splice site selection on transcript stability and translational efficiency are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Transcript accumulation and utilization of alternate and non-consensus splice sites in rice granule-bound starch synthase are temperature-sensitive and controlled by a single-nucleotide polymorphism

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1999 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1006298608408
Publisher site
See Article on Publisher Site

Abstract

Granule-bound starch synthase (GBSS), a product of the waxy gene in rice (Oryza sativa L.), is necessary for the synthesis of amylose in the endosperm. In an extended pedigree of 89 rice cultivars, we have previously shown that all cultivars with more than 18% amylose had the sequence AGGTATA at the leader intron 5′ splice site, while all cultivars with a lower proportion of amylose had the sequence AGTTATA. This single-nucleotide polymorphism reduces the efficiency of GBSS pre-mRNA processing. It also results in alternate splicing at multiple sites, some of which have non-consensus sequences. Here we demonstrate that this same G-to-T polymorphism is also associated with differential sensitivity to temperature during the period of grain development. Cultivars with the sequence AGTTATA have a substantial increase in accumulation of mature GBSS transcripts at 18 °C compared to 25 or 32 °C. The selection of leader intron 5′ splice sites is also affected by temperature in these cultivars. A 5′ splice site −93 upstream from that used in high-amylose varieties predominates at 18 °C. At higher temperatures there is increased utilization of a 5′ splice site at −1 and a non-consensus site at +1. Potential implications of differential 5′ splice site selection and associated differences in 3′ splice site selection on transcript stability and translational efficiency are discussed.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 19, 2004

References

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