Mammalian Genome 11, 598–602 (2000). DOI: 10.1007/s003350010114 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 Toward the yeastification of mouse genetics: chemical mutagenesis of embryonic stem cells 1 2 1 Yijing Chen, John Schimenti, Terry Magnuson Department of Genetics, Case Western Reserve University, 10900 Euclid Ave., Cleveland, Ohio 44106-4955, USA The Jackson Laboratory, Bar Harbor, Maine 04609, USA Received: 18 February 2000 / Accepted: 16 March 2000 Introduction X-linked hypoxanthine phosphoribosyl transferase (Hprt) coding region regardless of their cellular phenotypes (Chen et al. 2000). Functional genetic analysis requires mutations. Mutagenesis has When considering the full spectrum of mutations, the frequency long been a fundamental genetic tool for the analysis of experi- was approximately 1 mutation per 200 mutagenized clones (Chen mentally tractable organisms such as yeast, fruit flies, and nema- et al. 2000). Half of the mutations identified showed 6-thioguanine todes. Large-scale mutagenesis programs have been essential for (6-TG) resistance, a loss-of-function Hprt phenotype consistent the identification of genes controlling cellular and developmental with the non-conservative missense and splicing mutations re- pathways in these organisms and have provided a wealth of mu- vealed by sequence analysis. The remaining clones that were 6-TG tants in the vertebrate Danio rerio
Mammalian Genome – Springer Journals
Published: Feb 25, 2014
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