Toward the yeastification of mouse genetics: chemical mutagenesis of embryonic stem cells

Toward the yeastification of mouse genetics: chemical mutagenesis of embryonic stem cells Mammalian Genome 11, 598–602 (2000). DOI: 10.1007/s003350010114 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2000 Toward the yeastification of mouse genetics: chemical mutagenesis of embryonic stem cells 1 2 1 Yijing Chen, John Schimenti, Terry Magnuson Department of Genetics, Case Western Reserve University, 10900 Euclid Ave., Cleveland, Ohio 44106-4955, USA The Jackson Laboratory, Bar Harbor, Maine 04609, USA Received: 18 February 2000 / Accepted: 16 March 2000 Introduction X-linked hypoxanthine phosphoribosyl transferase (Hprt) coding region regardless of their cellular phenotypes (Chen et al. 2000). Functional genetic analysis requires mutations. Mutagenesis has When considering the full spectrum of mutations, the frequency long been a fundamental genetic tool for the analysis of experi- was approximately 1 mutation per 200 mutagenized clones (Chen mentally tractable organisms such as yeast, fruit flies, and nema- et al. 2000). Half of the mutations identified showed 6-thioguanine todes. Large-scale mutagenesis programs have been essential for (6-TG) resistance, a loss-of-function Hprt phenotype consistent the identification of genes controlling cellular and developmental with the non-conservative missense and splicing mutations re- pathways in these organisms and have provided a wealth of mu- vealed by sequence analysis. The remaining clones that were 6-TG tants in the vertebrate Danio rerio http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Toward the yeastification of mouse genetics: chemical mutagenesis of embryonic stem cells

Loading next page...
 
/lp/springer_journal/toward-the-yeastification-of-mouse-genetics-chemical-mutagenesis-of-f2Bfln0nAl
Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003350010114
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial