Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927) regenerants

Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp.... Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5 % of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927) regenerants

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Publisher
Springer Journals
Copyright
Copyright © 2015 by The Author(s)
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0368-0
Publisher site
See Article on Publisher Site

Abstract

Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5 % of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 3, 2015

References

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    Alhani, MC; Wilkinson, MJ
  • The effect of colchicine on triticale anther-derived plants: microspore pre-treatment and haploid-plant treatment using a hydroponic recovery system
    Arzani, A; Darvey, N
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    Bairu, M; Aremu, A; Staden, J
  • DNA-methylation changes in grapevine somaclones following in vitro culture and thermotherapy
    Baránek, M; Křižan, B; Ondrušíková, E; Pidra, M
  • Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers
    Bhatia, R; Singh, KP; Jhang, T; Sharma, TR
  • Measurement of the extent of somaclonal variation in begonia plants regenerated under various conditions: comparison of three assays
    Bouman, H; Klerk, G-J
  • Heritable somaclonal variation in wild barley (Hordeum spontaneum)
    Breiman, A; Rotem-Abarbanell, D; Karp, A; Shaskin, H
  • Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection
    Dann, A; Wilson, C
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    Gao, X; Yang, D; Cao, D; Ao, M; Sui, X; Wang, Q; Kimatu, JN; Wang, L
  • Tissue culture-induced locus-specific alteration in DNA methylation and its correlation with genetic variation in Codonopsis lanceolata Benth. et Hook. f
    Guo, WL; Wu, R; Zhang, YF; Liu, XM; Wang, HY; Gong, L; Zhang, ZH; Liu, B
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    Hossain, AM; Konisho, K; Mineo, M; Kazuhiro, N
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  • Production of haploids in bread wheat, durum wheat and hexaploid triticale crossed with pearl millet
    Inagaki, MN; Hash, CT

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