Pharm Res (2018) 35:155 https://doi.org/10.1007/s11095-018-2431-4 RESEARCH PAPER Three-Dimensional Printing of Cell Exclusion Spacers (CES) for Use in Motility Assays 1 2 1 1 3 1 Christen J. Boyer & David H. Ballard & Jungmi W. Yun & Adam Y. Xiao & Jeffery A. Weisman & Mansoureh Barzegar & Jonathan Steven Alexander Received: 31 March 2018 /Accepted: 15 May 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 ABSTRACT studying cell motility, migration/invasion, and complex Purpose Cell migration/invasion assays are widely used in multi-cell interactions. commercial drug discovery screening. 3D printing enables . . the creation of diverse geometric restrictive barrier designs KEY WORDS 3D printing invasion assays migration . . . for use in cell motility studies, permitting on-demand assays. assays motility assays personalized medicine Here, the utility of 3D printed cell exclusion spacers (CES) was three-dimensional printing validated as a cell motility assay. Methods A novel CES fit was fabricated using 3D printing and customized to the size and contour of 12 cell culture plates ABBREVIATIONS including 6 well plates of basal human brain vascular endo- CES Cell exclusion spacers thelial (D3) cell migration cells compared with 6 well plates Cyto-D Cytochalasin D with D3 cells
Pharmaceutical Research – Springer Journals
Published: Jun 4, 2018
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