Three-dimensional micro-PTV using deconvolution microscopy

Three-dimensional micro-PTV using deconvolution microscopy A three-dimensional micro-particle tracking velocimetry (micro-PTV) scheme is presented using a single camera with deconvolution microscopy. This method devises tracking of the line-of-sight (z) flow vectors by correlating the diffraction pattern ring size variations with the defocusing distances of small particle locations. The working principle is based on optical serial sectioning microscopy, or equivalently deconvolution microscopy, that records images of an infinitesimally small particle, and generates a point-spread function of the three-dimensional diffraction patterns. A new image-processing algorithm has also been developed to digitally identify the center locations and measure the radii of the diffraction rings, which allows simultaneous tracking of all three-vector components. The developed PTV technique uses a 40×, 0.75 NA dry objective lens with 500-nm fluorescent seeding particles of SG=1.05, and successfully measures the fully three-dimensional fields flowing over a spherical obstacle snuggly fitted inside a 100 μm × 100 μm micro-channel. The volumetric measurement resolution of the present system is equivalent to a 5.16 μm × 5.16 μm × 5.16 μm cube, and the overall measurement uncertainty for single-point velocity vector detection is estimated to ±7.58%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Three-dimensional micro-PTV using deconvolution microscopy

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Publisher
Springer-Verlag
Copyright
Copyright © 2005 by Springer-Verlag
Subject
Engineering; Engineering Fluid Dynamics; Fluid- and Aerodynamics; Engineering Thermodynamics, Heat and Mass Transfer
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s00348-005-0090-9
Publisher site
See Article on Publisher Site

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