Three-dimensional fluorescent particle tracking at micron-scale using a single camera

Three-dimensional fluorescent particle tracking at micron-scale using a single camera This article reports a new approach to track (x, y, z, t) coordinates of multiple fluorescent particles (diameter range 1–10 μm) simultaneously using a quantitative defocusing method. We find that the defocused image of a 1-μm diameter fluorescent particle formed by the objective lens of a conventional microscope has a bright outer ring due to the spherical aberration of the lens system. The ring radius increases as the particle is moved away from its reference plane and closer to the lens. The reference plane refers to locations of the particle at which the projected image is in focus. The (x, y, z) coordinates of the particle are then inferred from the center location of the image ring as well as the ring radius. The described technique is implemented successfully for obtaining 3D trajectories of swimming Escherichia coli cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Three-dimensional fluorescent particle tracking at micron-scale using a single camera

Loading next page...
 
/lp/springer_journal/three-dimensional-fluorescent-particle-tracking-at-micron-scale-using-UshaA9ILBQ
Publisher
Springer-Verlag
Copyright
Copyright © 2005 by Springer-Verlag
Subject
Engineering
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s00348-004-0925-9
Publisher site
See Article on Publisher Site

Abstract

This article reports a new approach to track (x, y, z, t) coordinates of multiple fluorescent particles (diameter range 1–10 μm) simultaneously using a quantitative defocusing method. We find that the defocused image of a 1-μm diameter fluorescent particle formed by the objective lens of a conventional microscope has a bright outer ring due to the spherical aberration of the lens system. The ring radius increases as the particle is moved away from its reference plane and closer to the lens. The reference plane refers to locations of the particle at which the projected image is in focus. The (x, y, z) coordinates of the particle are then inferred from the center location of the image ring as well as the ring radius. The described technique is implemented successfully for obtaining 3D trajectories of swimming Escherichia coli cells.

Journal

Experiments in FluidsSpringer Journals

Published: Feb 26, 2005

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off