Thermocouple psychrometer for measurements of water potential in plant tissues by isopiestic method

Thermocouple psychrometer for measurements of water potential in plant tissues by isopiestic method The paper describes a thermocouple psychrometer for measurements of water potential (ψw) and its components—osmotic potential (ψs + m) and turgor pressure (ψp)—in biological objects. The isopiestic method applied in this work does not require preliminary scarification of plant material for eliminating cuticular resistance to diffusion of water vapors. The device is reliable and simple in operation owing to an original design of replaceable plungers carrying the thermocouples. A modified construction of the lid for a thermocouple chamber and the application of a cryoholder excluded the necessity of removing the sample from the chamber after ψw measurements prior to its freezing in liquid nitrogen and subsequent thawing for determination of ψs +m. This feature improves the accuracy of determining ψp, which is calculated as ψw − ψs + m. The device can operate with minimal quantities of plant material and allows determination of all three components (ψw, ψs + m, ψp) for the same sample. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Thermocouple psychrometer for measurements of water potential in plant tissues by isopiestic method

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710050171
Publisher site
See Article on Publisher Site

Abstract

The paper describes a thermocouple psychrometer for measurements of water potential (ψw) and its components—osmotic potential (ψs + m) and turgor pressure (ψp)—in biological objects. The isopiestic method applied in this work does not require preliminary scarification of plant material for eliminating cuticular resistance to diffusion of water vapors. The device is reliable and simple in operation owing to an original design of replaceable plungers carrying the thermocouples. A modified construction of the lid for a thermocouple chamber and the application of a cryoholder excluded the necessity of removing the sample from the chamber after ψw measurements prior to its freezing in liquid nitrogen and subsequent thawing for determination of ψs +m. This feature improves the accuracy of determining ψp, which is calculated as ψw − ψs + m. The device can operate with minimal quantities of plant material and allows determination of all three components (ψw, ψs + m, ψp) for the same sample.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Sep 2, 2010

References

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