Theoretical and experimental analysis of the accuracy and reproducibility of cell tracking velocimetry

Theoretical and experimental analysis of the accuracy and reproducibility of cell tracking... A theoretical and experimental analysis has been conducted to determine the accuracy of cell tracking velocimetry (CTV). CTV is an analytical technique for quantifying magnetically induced velocity of immunomagnetically labeled cells (or particles), in which the computer algorithm, particle tracking velocimetry (PTV), has been modified and combined with a well-defined magnetic energy gradient. In addition, this technique can calculate the size of a cell (or particle) through the use of experimentally measured settling velocities. A model was developed which determines the minimum and maximum cell velocities that can be determined based on a number of intrinsic constants and variables associated with this technique. This model was experimentally tested using a number of calibration particles and very good agreement between model and experimental data was obtained. The combination of model and experimental validation establishes the proper operating parameters for CTV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Theoretical and experimental analysis of the accuracy and reproducibility of cell tracking velocimetry

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Publisher
Springer-Verlag
Copyright
Copyright © 2001 by Springer-Verlag Berlin Heidelberg
Subject
Engineering; Engineering Fluid Dynamics; Fluid- and Aerodynamics; Engineering Thermodynamics, Heat and Mass Transfer
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s003480000211
Publisher site
See Article on Publisher Site

Abstract

A theoretical and experimental analysis has been conducted to determine the accuracy of cell tracking velocimetry (CTV). CTV is an analytical technique for quantifying magnetically induced velocity of immunomagnetically labeled cells (or particles), in which the computer algorithm, particle tracking velocimetry (PTV), has been modified and combined with a well-defined magnetic energy gradient. In addition, this technique can calculate the size of a cell (or particle) through the use of experimentally measured settling velocities. A model was developed which determines the minimum and maximum cell velocities that can be determined based on a number of intrinsic constants and variables associated with this technique. This model was experimentally tested using a number of calibration particles and very good agreement between model and experimental data was obtained. The combination of model and experimental validation establishes the proper operating parameters for CTV.

Journal

Experiments in FluidsSpringer Journals

Published: Apr 4, 2001

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