Mammalian Genome 9, 676–678 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 The Xt allele generates a Gli3 fusion transcript Dirk Bu ¨ scher,*,** Lars Grotewold,* Ulrich Ru ¨ ther Institut fu ¨ r Molekularbiologie, Medizinische Hochschule Hannover OE 5251, 30623 Hannover, Germany Received: 20 February 1998 / Accepted: 6 April 1998 The semidominant mouse mutant extra toes (Xt) is mainly char- sequence of 293 bp fused to Gli3 at position 1670 (Fig. 2a). The acterized by its abnormal limb and brain structures (Johnson existence of this fusion transcript was confirmed in Xt/+ and Xt/Xt 1967). Heterozygous Xt/+ mice show preaxial polydactyly, embryos by RT-PCR (Fig. 2b) and RNase protection analysis (not whereas homozygous Xt/Xt embryos die around birth, exhibiting shown). Since we could not amplify a fragment from wild-type severe craniofacial abnormalities often combined with exen- RNA using the primer combination specific for Gli3 and the novel cephaly and an enhanced polydactyly with up to nine digits. sequence (Fig. 2b), we can exclude that this sequence is part of the The gene affected by this mutation is Gli3, which is coding for 38 UTR of Gli3. Thus, the deletion extends beyond the 38 end of a
Mammalian Genome – Springer Journals
Published: Aug 1, 1998
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