The Usage of Plasmalemmal Vesicles Inverted by Brij 58 Treatment for Studying Processes, which Occur on the Cytosolic Membrane Surface

The Usage of Plasmalemmal Vesicles Inverted by Brij 58 Treatment for Studying Processes, which... We studied the possible use of the detergent Brij 58 in physiological experiments for the reorientation of right-side-out plasmalemmal vesicles, which were isolated from wheat (Triticum aestivum L.) coleoptiles. The activities of K+, Mg2+-ATPase and the ATP-dependent H+-potential were higher in Brij 58-treated vesicles, whereas membrane permeability for K+ and Na+ remained unchanged. Brij 58 did not suppress the ATP-dependent IAA transport into vesicles and RNA polymerase II activation by IAA–protein plasmalemmal complexes in the system of isolated nuclei. The conclusion was that, using Brij 58, we could obtain the plasmalemmal fraction, which consisted almost completely of closed inverted vesicles. These vesicles can be applied for the in vitro study of the processes, which occur on the cytosolic plasmalemmal surface. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The Usage of Plasmalemmal Vesicles Inverted by Brij 58 Treatment for Studying Processes, which Occur on the Cytosolic Membrane Surface

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1020957427426
Publisher site
See Article on Publisher Site

Abstract

We studied the possible use of the detergent Brij 58 in physiological experiments for the reorientation of right-side-out plasmalemmal vesicles, which were isolated from wheat (Triticum aestivum L.) coleoptiles. The activities of K+, Mg2+-ATPase and the ATP-dependent H+-potential were higher in Brij 58-treated vesicles, whereas membrane permeability for K+ and Na+ remained unchanged. Brij 58 did not suppress the ATP-dependent IAA transport into vesicles and RNA polymerase II activation by IAA–protein plasmalemmal complexes in the system of isolated nuclei. The conclusion was that, using Brij 58, we could obtain the plasmalemmal fraction, which consisted almost completely of closed inverted vesicles. These vesicles can be applied for the in vitro study of the processes, which occur on the cytosolic plasmalemmal surface.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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