The Transplantation of hBM-MSCs Increases Bone Neo-Formation and Preserves Hearing Function in the Treatment of Temporal Bone Defects – on the Experience of Two Month Follow Up

The Transplantation of hBM-MSCs Increases Bone Neo-Formation and Preserves Hearing Function in... Temporal bone reconstruction is a persisting problem following middle ear cholesteatoma surgery. Seeking to advance the clinical transfer of stem cell therapy we attempted the reconstruction of temporal bone using a composite bioartificial graft based on a hydroxyapatite bone scaffold combined with human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). The aim of this study was to evaluate the effect of the combined biomaterial on the healing of postoperative temporal bone defects and the preservation of physiological hearing functions in a guinea pig model. The treatment’s effect could be observed at 1 and 2 months after implantation of the biomaterial, as opposed to the control group. The clinical evaluation of our results included animal survival, clinical signs of an inflammatory response, and exploration of the tympanic bulla. Osteogenesis, angiogenesis, and inflammation were evaluated by histopathological analyses, whereas hBM-MSCs survival was evaluated by immunofluorescence assays. Hearing capacity was evaluated by objective audiometric methods, i.e. auditory brainstem responses and otoacoustic emission. Our study shows that hBM-MSCs, in combination with hydroxyapatite scaffolds, improves the repair of bone defects providing a safe and effective alternative in their treatment following middle ear surgery due to cholesteatoma. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Stem Cell Reviews and Reports Springer Journals

The Transplantation of hBM-MSCs Increases Bone Neo-Formation and Preserves Hearing Function in the Treatment of Temporal Bone Defects – on the Experience of Two Month Follow Up

Download PDF
11 pages
Read Article

Loading next page...
 
/lp/springer_journal/the-transplantation-of-hbm-mscs-increases-bone-neo-formation-and-XioBR0q9d0
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Life Sciences; Stem Cells; Regenerative Medicine/Tissue Engineering; Cell Biology; Biomedical Engineering
ISSN
1550-8943
eISSN
1558-6804
DOI
10.1007/s12015-018-9831-z
Publisher site
See Article on Publisher Site

Abstract

Temporal bone reconstruction is a persisting problem following middle ear cholesteatoma surgery. Seeking to advance the clinical transfer of stem cell therapy we attempted the reconstruction of temporal bone using a composite bioartificial graft based on a hydroxyapatite bone scaffold combined with human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). The aim of this study was to evaluate the effect of the combined biomaterial on the healing of postoperative temporal bone defects and the preservation of physiological hearing functions in a guinea pig model. The treatment’s effect could be observed at 1 and 2 months after implantation of the biomaterial, as opposed to the control group. The clinical evaluation of our results included animal survival, clinical signs of an inflammatory response, and exploration of the tympanic bulla. Osteogenesis, angiogenesis, and inflammation were evaluated by histopathological analyses, whereas hBM-MSCs survival was evaluated by immunofluorescence assays. Hearing capacity was evaluated by objective audiometric methods, i.e. auditory brainstem responses and otoacoustic emission. Our study shows that hBM-MSCs, in combination with hydroxyapatite scaffolds, improves the repair of bone defects providing a safe and effective alternative in their treatment following middle ear surgery due to cholesteatoma.

Journal

Stem Cell Reviews and Reports – Springer Journals

Published: Jun 3, 2018

References

  • Tolerance and osteointegration of TricOs(TM)/MBCP(®) in association with fibrin sealant in mastoid obliteration after canal wall-down technique for cholesteatoma
    Franco-Vidal, K; Daculsi, G; Bagot d’Arc, M; Sterkes, O; Smail, M; Robier, A; Bordure, P; Claros, P; Paiva, A; Darrouzet, V; Anthoine, E; Bedear, JP
  • Partial mastoid obliteration combined with soft-wall reconstruction for modele ear cholesteatoma
    Suzuki, H; Ikezaki, S; Imazato, K; Koizumi, H; Ohbuchi, T; Hohchi, N; Hashida, K
  • Pooled analysis of the evidence for open cavity, combined approach and reconstruction of the mastoid cavity in primary cholesteatoma surgery
    Harris, AT; Mettias, B; Lesser, TH
  • Stem cells in bone tissue engineering
    Seong, JM; Kim, BC; Park, JH; Kwon, IK; Mantalaris, A; Hwang, YS
  • Skeletal tissue engineering using mesenchymal or embryonic stem cells: clinical and experimental data
    Gamie, Z; MacFarlane, RJ; Tomkinson, A; Moniakis, A; Tran, GT; Gamie, Y; Mantalaris, A; Tsiridis, E
  • Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
    Dominici, M; Blanc, K; Mueller, I; Slaper-Cortenbach, I; Marini, F; Krause, D; Deans, R; Keating, A; Prockop, DJ; Horwitz, E
  • Mesenchymal stem cells and their use therapy: what has been achieved?
    Fernández Vallone, VB; Romaniuk, MA; Choi, H; Labovsky, V; Otaegui, J; Chasseing, NA
  • Multipotent stromal cells for autologous cell therapy approaches in the Guinea pig model
    Frölich, K; Scheryed, A; Mlynski, R; Technau, A; Hagen, R; Kleinsasser, N; Radeloff, A
  • The use of laryngeal mucosa mesenchymal stem cells for the repair the vocal fold injury
    Peng, H; Ming, L; Yang, R; Liu, Y; Liang, Y; Zhao, Y; Jin, Y; Deng, Z
  • Multipotent mesenchymal stromal cells in otorhinolaryngology
    Skoloudik, Lukas; Chrobok, Viktor; Kalfert, David; Koci, Zuzana; Filip, Stanislav
  • Human multipotent mesenchymal stroma cells in the treatment of postoperative temporal bone defect: an animal model
    Skoloudik, L; Chrobok, V; Kalfert, D; Koci, Z; Sykova, E; Chumak, T; Popelar, J; Syka, J; Laco, J; Dedkova, J; Dayanithi, G; Filip, S
  • The osteogenic potential of human nondifferentiated and pre-differentiated mesenchymal stem cells combined with an osteoconductive scaffold – early stage healing
    Tuček, L; Kočí, Z; Kárová, K; Doležalová, H; Suchánek, J
  • A study of metabolites as intermediate effectors in angiogenesis
    Murray, B; Wilson, DJ
  • Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosis
    Boomsma, RA; Geenen, DL
  • In vitro augmentation of mesenchymal stem cells viability in stressful microenvironments: In vitro augmentation of mesenchymal stem cells viability
    Amiri, F; Jahanian-Najafabadi, A; Roudkenar, MH
  • The protection of MSCs from apoptosis in nerve regeneration by TGFβ1 through reducing inflammation and promoting VEGF-dependent angiogenesis
    Luo, H; Zhang, Y; Zhang, Z; Jin, Y
  • A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods
    Madrigal, M; Rao, KS; Riordan, NH
  • Bone marrow derived stem cells in trauma and ortophedics: a review of the current trend
    Singh, J; Onimowo, JO; Khan, WS
  • In vivo therapeutic potential of mesenchymal stromal cells depends on the source and the isolation procedure
    Bortolotti, F; Ukovich, L; Razban, V; Martinelli, V; Ruozi, G; Pelos, B; Dore, F; Giacca, M; Zaschigna, S
  • Clinical trials with mesenchymal stem cells: an update
    Squillaro, T; Peluso, G; Galderisi, U
  • The combination of mesenchymal stem cells a bone scaffold in the treatement of vertebral body defects
    Vanecek, V; Klima, K; Kohout, A; Foltan, R; Jirousek, O; Sedy, J; Stulik, J; Sykova, E; Jendelova, P
  • Topographical anatomy of the Guinea pig temporal bone
    Wysocki, J
  • Properties and growth of human bone marrow mesenchymal stromal cells cultivated in different media
    Truncova, K; Ruyickova, K; Vanecek, V; Sykova, E; Jendelova, P
  • Bioactive glass ceramic particles as an alternative for mastoid obliteration: results in an animal model
    Leatherman, BD; Dornhoffer, JL
  • Mesenchymal and hematopoietic stem cells from a unique bone marrow niche
    Mendez-Ferrer, S; Michurina, TV; Ferrari, F; Maylom, AR; Macarthur, BD; Lira, SA; Scadden, DT; Ma’ayan, A; Enikopolov, GN; Frenette, PS
  • Clinical significance of β-tricalcium phosphate and polyphosphate for mastoid cavity obliteration during middle ear surgery: human and animal study
    Lee, HB; Lim, HJ; Cho, M; Yang, SM; Park, K; Park, H-Y; Choung, YH
  • Natural history of mesenchymal stem cells, from vessel walls to culture vessel
    Murray, IR; West, C; Hardy, WR; James, AW; Park, TS; Nguyen, A; Tawonsawatruk, T; Lazzari, L; Soo, C; Péault, B
  • Osteogenesis for postoperative temporal bone defects using human ear adipose-derived stromal cells and tissue engineering: an animal model study
    Kim, YJ; Park, SG; Shin, B; Kim, J; Kim, SW; Choo, OS; Yin, XY; Min, BH; Choung, YH
  • Effects of epigalloctechin gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline
    Kashiwa, K; Kotobiki, N; Tadokoro, M; Matsumura, K; Hyon, SH; Yoshiya, S; Ohgushi, H
  • Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration
    Jang, CH; Cho, YB; Choi, CH; Jang, YS; Jung, WK; Lee, H; Kim, GH
  • Immunomodulatory activity of mesenchymal stem cells
    Kaplan, JM; Youd, ME; Lodie, TA
  • Endothelial progenitor cells improve directly and indirectly early vascularization of mesenchymal stem cell derived bone regeneration in a critical bone defect in rats
    Seebach, C; Henrich, D; Wilhelm, K; Barker, JH; Marzi, I

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

Sign up
for free
Start 14 day
Free Trial