The Temperature Dependence of Intracellular pH in Isolated Frog Skeletal Muscle: Lessons Concerning the Na+-H+ Exchanger

The Temperature Dependence of Intracellular pH in Isolated Frog Skeletal Muscle: Lessons... We used 31P NMR to investigate the temperature-dependence of intracellular pH (pH i ) in isolated frog skeletal muscles. We found that ln[H+ i ] is a linear function of 1/T abs paralleling those of neutral water (i.e., H+= OH−) and of a solution containing the fixed pH buffers of frog muscle cytosol. This classical van't Hoff relationship was unaffected by inhibition of glycolysis and was not dependent upon the pH or [Na+] in the bathing solution. Insulin stimulation of Na+-H+ exchange shifted the intercept in the alkaline direction but had no effect on the slope. Acid loading followed by washout resulted in an amiloride-sensitive return to the (temperature dependent) basal pH i . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

The Temperature Dependence of Intracellular pH in Isolated Frog Skeletal Muscle: Lessons Concerning the Na+-H+ Exchanger

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 1998 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002329900328
Publisher site
See Article on Publisher Site

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