The role of Su(Hw) protein in transcription regulation in Drosophila melanogaster

The role of Su(Hw) protein in transcription regulation in Drosophila melanogaster Insulators are regulatory DNA elements that modulate the effect of enhancers and silencers on gene transcription. However, the role of the insulator proteins in the expression of specific genes remains scarcely studied. In the present study, we examined the role of the Su(Hw)-dependent insulator complex in transcription of the rap, CG32810, and RpS15Aa genes. It was demonstrated that the interaction of Su(Hw) and Mod(mdg4)-67.2 insulator proteins with the terminator regions of the selected genes depends on the Su(Hw) factor. At the same time, the CP190 protein interacts with the promoter and terminator regions in the absence of Su(Hw). In addition, the Su(Hw) protein does not affect the level and efficiency of termination of model genes transcription. A possible explanation for these results is the existence of another transcription factor, a protein that is able to recruit CP190 and functionally compensate for the lack of the Su(Hw) protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

The role of Su(Hw) protein in transcription regulation in Drosophila melanogaster

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795415110058
Publisher site
See Article on Publisher Site

Abstract

Insulators are regulatory DNA elements that modulate the effect of enhancers and silencers on gene transcription. However, the role of the insulator proteins in the expression of specific genes remains scarcely studied. In the present study, we examined the role of the Su(Hw)-dependent insulator complex in transcription of the rap, CG32810, and RpS15Aa genes. It was demonstrated that the interaction of Su(Hw) and Mod(mdg4)-67.2 insulator proteins with the terminator regions of the selected genes depends on the Su(Hw) factor. At the same time, the CP190 protein interacts with the promoter and terminator regions in the absence of Su(Hw). In addition, the Su(Hw) protein does not affect the level and efficiency of termination of model genes transcription. A possible explanation for these results is the existence of another transcription factor, a protein that is able to recruit CP190 and functionally compensate for the lack of the Su(Hw) protein.

Journal

Russian Journal of Genetics – Springer Journals

Published: Nov 13, 2015

References

  • Making connections: insulators organize eukaryotic chromosomes into independent cis-regulatory networks
    Chetverina, D.; Aoki, T.; Erokhin, M.
  • Chromatin insulators and long-distance interactions in Drosophila
    Kyrchanova, O.; Georgiev, P.
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    Muravyova, E.; Golovnin, A.; Gracheva, E.
  • Effects on cis arrangement of chromatin insulators on enhancer-blocking activity
    Cai, H.; Shen, P.
  • Insulators form gene loops by interacting with promoters in Drosophila
    Erokhin, M.; Davydova, A.; Kyrchanova, O.
  • Effective blocking of the white enhancer requires cooperation between two main mechanisms suggested for the insulator function
    Kyrchanova, O.; Maksimenko, O.; Stakhov, V.
  • Study of long-distance functional interactions between Su(Hw) insulators that can regulate enhancer–promoter communication in Drosophila melanogaster
    Savitskaya, E.; Melnikova, L.; Kostuchenko, M.
  • Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements
    Kyrchanova, O.; Chetverina, D.; Maksimenko, O.
  • The Drosophila su(Hw) gene, which controls the phenotypic effect of the gypsy transposable element, encodes a putative DNA-binding protein
    Parkhurst, S.; Harrison, D.; Remington, M.
  • A leucine zipper domain of the suppressor of Hairy-wing protein mediates its repressive effect on enhancer function
    Harrison, D.; Gdula, D.; Coyne, R.; Corces, V.
  • The DNA-binding and enhancer-blocking domains of the Drosophila suppressor of Hairy-wing protein
    Kim, J.; Shen, B.; Rosen, C.; Dorsett, D.
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    Büchner, K.; Roth, P.; Schotta, G.
  • Insulation of enhancer-promoter communication by a gypsy transposon insert in the Drosophila cut gene: cooperation between suppressor of hairy-wing and modifier of mdg4 proteins
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    Ghosh, D.; Gerasimova, T.I.; Corces, V.G.
  • The centrosomal protein CP190 is a component of the gypsy chromatin insulator
    Pai, C.; Lei, E.; Ghosh, D.; Corces, V.
  • Integrity of the Mod(mdg4)-67.2 BTB domain is critical to insulator function in Drosophila melanogaster
    Golovnin, A.; Mazur, A.; Kopantseva, M.
  • CTCF-promoted RNA polymerase II pausing links DNA methylation to splicing
    Shukla, S.; Kavak, E.; Gregory, M.
  • The role of CTCF in regulating V(D)J recombination
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  • Insulator protein Su(Hw) recruits SAGA and Brahma complexes and constitutes part of Origin Recognition Complex-binding sites in the Drosophila genome
    Vorobyeva, N.; Mazina, M.; Golovnin, A.
  • 1A2 insulator can interact with promoter of hsp70 gene in D. melanogaster
    Chetverina, D.A.; Elizar’ev, P.V.; Georgiev, P.G.; Erokhin, M.M.
  • Genomic mapping of suppressor of Hairy-wing binding sites in Drosophila
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  • Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin
    Maksimenko, O.; Bartkuhn, M.; Stakhov, V.
  • Unravelling the means to an end: RNA polymerase II transcription termination
    Kuehner, J.; Pearson, E.; Moore, C.
  • The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning
    Mohan, M.; Bartkuhn, M.; Herold, M.
  • Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs
    Chetverina, D.; Savitskaya, E.; Maksimenko, O.

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