J. Plant Biol. (2017) 60:314-321
The Rice YGL Gene Encoding an Mg
-chelatase ChlD Subunit is Affected
by Temperature for Chlorophyll Biosynthesis
, Zhenyu Gao
, Juan Zhao
, Bin Zhang
, Anpeng Zhang
, Kai Hong
, Shenglong Yang
, Chaolei Liu
, Guang Chen
, Youlin Peng
, Guojun Dong
, Longbiao Guo
, Zhengjin Xu
and Qian Qian
Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and
Breeding, Ministry of Education and Liaoning Province/Key Laboratory of Northeast Rice Biology and Genetics and
Breeding, Ministry of Agriculture, Shenyang 110866, Liaoning, China
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China
Received: December 7, 2016 / Accepted: January 22, 2017
© Korean Society of Plant Biologists 2017
Abstract Leaf color-related mutants are important materials
to study the chlorophyll biosynthesis and chloroplast
development. Here, we isolated and characterized a thermo-
sensitive yellow-green leaf mutant, ygl, through mutagenized
Japonica rice cultivar Zhonghua11 by EMS. The mutant
showed yellow-green leaves only at the seedling stage.
Genetic and molecular analyses uncovered that YGL encode
-chelatase ChlD subunit, which was a new allele of
Chl1/YGL7. Quantitative PCR and GUS staining showed
that the YGL transcripts expressed in all tested organs and
subcellular localization detected YGL protein in the chloroplasts.
The mutant displayed yellow-green leaves at 20°C and
normal green leaves at 34°C during the seedling stage.
Transcript level of YGL in the ygl mutant was significantly
reduced at 20°C, and increased at 34°C compared with the
wild-type plant. Furthermore, the expression of chlorophyll
biosynthesis and chloroplast development related genes were
influenced by the mutation of YGL at different temperature.
These findings suggested that the YGL gene not only play a
key role in chlorophyll biosynthesis, but is affected at
transcript level by temperature in rice.
-chelatase ChlD subunit, Rice, Temperature
sensitivity, Yellow-green leaf
Leaf is the major photosynthetic organ of plants and the
main source of carbohydrates, and plays an important role in
crop yield and quality. Photosynthetic efficiency is closely
related to chlorophyll content, chloroplast morphology and
structure (Grossman et al. 1995). Leaf color-related mutants
are suitable materials to study chlorophyll biosynthesis and
chloroplast development, and the apparent phenotype of leaf
color can be used as a marker to identify hybrid rice.
To date, lots of leaf color associated genes have been
cloned in rice, including chlorophyll synthesis related genes,
such as YGL1, YGL2, OsCHLH, FGL, CHL1, CHL9, LYL1
and YLC1 (Wu et al. 2007; Chen et al. 2013; Jung et al.
2003; Sakuraba et al. 2013; Zhang et al. 2006; Zhou et al.
2012 and Zhou et al. 2013), chlorophyll degradation related
genes, such as NYC1, NYC3, SGR, and NOL (Kusaba et al,
2007; Morita et al. 2009; Jiang et al. 2007 and Sato et al,
2009), and chloroplast development related genes, such as
OsRpoTp, VYL, V1, V2, V3, OsPAPST1, OsCpn60α1, ETL1,
ETL2, OsDG2, OsHAP3A, TCD5 and YL1 (Miyoshi et al.
2003; Kusumi et al. 2004; Sugimoto et al. 2004; Yoo et al.
2009; Kusumi et al. 2011; Mao et al. 2011; Dong et al. 2013;
Xu et al. 2013; Kim et al. 2013; Jiang et al. 2014; Wang et
al. 2016 and Chen et al. 2016). Despite of functional
explanation for genes involved in leaf color mutation, the
complex biogenesis of the organelle has not been well
-chelatase is an enzyme for catalyzing Mg
into protoporphyrin IX to form Mg
(Bollivar et al. 1994). Mg
-chelatase consists of I (40-kDa),
D (70-kDa), and H (140-kDa) subunits (Davison et al. 2005).
These authors contributed equally to this work.
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