The Power of EPR Techniques in Investigating Functionalization and Penetration into Fibers of Cotton-Bound Antimicrobial Peptides

The Power of EPR Techniques in Investigating Functionalization and Penetration into Fibers of... The development of protective and safe textiles is of fundamental importance for defending the human body from bacterial infections. To this aim, garments are often functionalized with antibacterial agents. We recently started a program aimed at covalently linking antimicrobial peptides to cotton tissues. To optimize the process of binding, it is necessary to know the degree of functionalization and how deeply peptides penetrate into the cotton fiber. Here, we present a spin-label electron paramagnetic resonance (EPR) approach for obtaining data on the peptide incorporation into the fibers. The approach is based on the line broadening in conventional EPR and on the signal decays in electron spin echo spectroscopy that is a pulsed version of EPR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Magnetic Resonance Springer Journals

The Power of EPR Techniques in Investigating Functionalization and Penetration into Fibers of Cotton-Bound Antimicrobial Peptides

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag GmbH Austria
Subject
Physics; Solid State Physics; Spectroscopy/Spectrometry; Atoms and Molecules in Strong Fields, Laser Matter Interaction; Physical Chemistry; Organic Chemistry
ISSN
0937-9347
eISSN
1613-7507
D.O.I.
10.1007/s00723-017-0917-6
Publisher site
See Article on Publisher Site

Abstract

The development of protective and safe textiles is of fundamental importance for defending the human body from bacterial infections. To this aim, garments are often functionalized with antibacterial agents. We recently started a program aimed at covalently linking antimicrobial peptides to cotton tissues. To optimize the process of binding, it is necessary to know the degree of functionalization and how deeply peptides penetrate into the cotton fiber. Here, we present a spin-label electron paramagnetic resonance (EPR) approach for obtaining data on the peptide incorporation into the fibers. The approach is based on the line broadening in conventional EPR and on the signal decays in electron spin echo spectroscopy that is a pulsed version of EPR.

Journal

Applied Magnetic ResonanceSpringer Journals

Published: Jul 17, 2017

References

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