The point mutation induced by the low-energy N+ ion implantation in Impatiens balsamine genome

The point mutation induced by the low-energy N+ ion implantation in Impatiens balsamine genome To investigate the effect of genome mutations induced by low energy ions implantation in higher plants, genome mutation of Impatiens balsamine mutant induced by low energy N+ ion implantation were analyzed by the RAPD, ISSR and genome sequence. Six out of the 121 ISSR primers and 6 out of the 135 RAPD primers showed that polymorphism ratios between mutants and wild type were 4.96% and 2.89%, respectively. Sequence analysis revealed that base deletions, insertions, and substitutions were observed in the mutant genome comparable to wild type. N+ induced point mutations were mostly base substitution (77.4%), no duplication, long fragments insertions and deletions was found. In all point mutation, adenine (A) was most sensitive to the N+ ion implantation in impatiens. The transition was mainly A → guanine (G) (15.90%) and thymine (T) cytosine (C) (12.55%). Transversion happened in A ↔ T (16.74%), which much higher than C ↔ G (5.02%), G ↔ T (6.69%), A ↔ C (7.11%) bases. These findings indicate that low energy ions being a useful mutagen were mostly cause the point mutation in impatiens. Russian Journal of Genetics Springer Journals

The point mutation induced by the low-energy N+ ion implantation in Impatiens balsamine genome

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SP MAIK Nauka/Interperiodica
Copyright © 2012 by Pleiades Publishing, Ltd.
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
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