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C. Lico, Qiang Chen, L. Santi (2008)
Viral vectors for production of recombinant proteins in plantsJournal of Cellular Physiology, 216
Selvaraju Kanagarajan, Saraladevi Muthusamy, A. Gliszczyńska, Anneli Lundgren, P. Brodelius (2012)
Functional expression and characterization of sesquiterpene synthases from Artemisia annua L. using transient expression system in Nicotiana benthamianaPlant Cell Reports, 31
A. Kusnadi, Z. Nikolov, J. Howard (1997)
Production of recombinant proteins in transgenic plants: Practical considerationsBiotechnology and Bioengineering, 56
M. Cañizares, Li Liu, Y. Perrin, E. Tsakiris, G. Lomonossoff (2006)
A bipartite system for the constitutive and inducible expression of high levels of foreign proteins in plants.Plant biotechnology journal, 4 2
Qiao-Yang Sun, L. Ding, G. Lomonossoff, Yonghong Sun, M. Luo, Chaoqiong Li, Liwen Jiang, Zeng-Fu Xu (2011)
Improved expression and purification of recombinant human serum albumin from transgenic tobacco suspension culture.Journal of biotechnology, 155 2
N Bechtold, G Pelletier (1998)
Arabidopsis protocols
J. Rohll, C. Holness, G. Lomonossoff, A. Maule (1993)
3'-terminal nucleotide sequences important for the accumulation of cowpea mosaic virus M-RNA.Virology, 193 2
A. Giritch, S. Marillonnet, Carola Engler, G. Eldik, J. Botterman, V. Klimyuk, Y. Gleba (2006)
Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectorsProceedings of the National Academy of Sciences, 103
G. Pogue, J. Lindbo, S. Garger, W. Fitzmaurice (2002)
Making an ally from an enemy: plant virology and the new agriculture.Annual review of phytopathology, 40
H. Scholthof, K. Scholthof, A. Jackson (1996)
Plant virus gene vectors for transient expression of foreign proteins in plants.Annual review of phytopathology, 34
Jeffrey Larsen, W. Curtis (2012)
RNA viral vectors for improved Agrobacterium-mediated transient expression of heterologous proteins in Nicotiana benthamiana cell suspensions and hairy rootsBMC Biotechnology, 12
Frank Sainsbury, P. Saxena, K. Geisler, A. Osbourn, G. Lomonossoff (2012)
Using a virus-derived system to manipulate plant natural product biosynthetic pathways.Methods in enzymology, 517
Frank Sainsbury, G. Lomonossoff (2008)
Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication1Plant Physiology, 148
N. Bechtold, G. Pelletier (1998)
In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.Methods in molecular biology, 82
(2013)
Plant Mol Biol
Li Liu, G. Lomonossoff (2002)
Agroinfection as a rapid method for propagating Cowpea mosaic virus-based constructs.Journal of virological methods, 105 2
B. Harrison, A. Murant (1996)
Polyhedral virions and bipartite RNA genomes
M. Vardakou, Frank Sainsbury, N. Rigby, F. Mulholland, G. Lomonossoff (2012)
Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system.Protein expression and purification, 81 1
S. Matić, Riccardo Rinaldi, V. Masenga, E. Noris (2011)
Efficient production of chimeric Human papillomavirus 16 L1 protein bearing the M2e influenza epitope in Nicotiana benthamiana plantsBMC Biotechnology, 11
Selvaraju Kanagarajan, C. Tolf, Anneli Lundgren, J. Waldenström, P. Brodelius (2012)
Transient Expression of Hemagglutinin Antigen from Low Pathogenic Avian Influenza A (H7N7) in Nicotiana benthamianaPLoS ONE, 7
S. Canaan, L. Dupuis, M. Rivière, K. Faessel, J. Romette, R. Verger, C. Wicker-Planquart (1998)
Purification and interfacial behavior of recombinant human gastric lipase produced from insect cells in a bioreactor.Protein expression and purification, 14 1
Frank Sainsbury, M. Sack, J. Stadlmann, Heribert Quendler, R. Fischer, G. Lomonossoff (2010)
Rapid Transient Production in Plants by Replicating and Non-Replicating Vectors Yields High Quality Functional Anti-HIV AntibodyPLoS ONE, 5
E. Thuenemann, P. Lenzi, A. Love, M. Taliansky, M. Bécares, S. Zúñiga, L. Enjuanes, G. Zahmanova, I. Minkov, S. Matić, E. Noris, A. Meyers, Alta Hattingh, E. Rybicki, O. Kiselev, N. Ravin, M. Eldarov, K. Skryabin, G. Lomonossoff (2013)
The use of transient expression systems for the rapid production of virus-like particles in plants.Current pharmaceutical design, 19 31
Frank Sainsbury , K. Saunders, A. Aljabali, David Evans, G. Lomonossoff (2011)
Peptide‐Controlled Access to the Interior Surface of Empty Virus NanoparticlesChemBioChem, 12
K. Miyamoto, Takafumi Shimizu, Fengqiu Lin, Frank Sainsbury, E. Thuenemann, G. Lomonossoff, H. Nojiri, H. Yamane, K. Okada (2012)
Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice.Journal of plant physiology, 169 6
Frank Sainsbury, M. Cañizares, G. Lomonossoff (2010)
Cowpea mosaic virus: the plant virus-based biotechnology workhorse.Annual review of phytopathology, 48
R. Goldbach, J. Wellink (1996)
Comoviruses: Molecular Biology and Replication
S. Matić, V. Masenga, A. Poli, Riccardo Rinaldi, R. Milne, M. Vecchiati, E. Noris (2012)
Comparative analysis of recombinant Human Papillomavirus 8 L1 production in plants by a variety of expression systems and purification methods.Plant biotechnology journal, 10 4
Frank Sainsbury, E. Thuenemann, G. Lomonossoff (2009)
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants.Plant biotechnology journal, 7 7
J. Lindbo (2007)
TRBO: A High-Efficiency Tobacco Mosaic Virus RNA-Based Overexpression Vector1[C][OA]Plant Physiology, 145
K. Saunders, Frank Sainsbury, G. Lomonossoff (2009)
Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants.Virology, 393 2
M. Cañizares, L. Nicholson, G. Lomonossoff (2005)
Use of viral vectors for vaccine production in plantsImmunology and Cell Biology, 83
P. Saxena, Y. Hsieh, Veria Alvarado, Frank Sainsbury, K. Saunders, G. Lomonossoff, H. Scholthof (2011)
Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless.Plant biotechnology journal, 9 6
Nicholas Montague, E. Thuenemann, P. Saxena, K. Saunders, P. Lenzi, G. Lomonossoff (2011)
Recent advances of Cowpea mosaic virus-based particle technologyHuman Vaccines, 7
Marc‐André D'Aoust, M. Couture, N. Charland, S. Trépanier, N. Landry, Frédéric Ors, L. Vézina (2010)
The production of hemagglutinin-based virus-like particles in plants: a rapid, efficient and safe response to pandemic influenza.Plant biotechnology journal, 8 5
H. Meijer, Adri Thomas (2002)
Control of eukaryotic protein synthesis by upstream open reading frames in the 5'-untranslated region of an mRNA.The Biochemical journal, 367 Pt 1
T. Crabbe, A. Weir, E. Walton, M. Brown, C. Sutton, N. Trétout, J. Bonnerjea, P. Lowe, G. Yarranton (1996)
The secretion of active recombinant human gastric lipase by Saccharomyces cerevisiae.Protein expression and purification, 7 3
J. Grainger (1946)
Plant VirusesNature, 158
A. Love, S. Chapman, S. Matić, E. Noris, G. Lomonossoff, M. Taliansky (2012)
In planta production of a candidate vaccine against bovine papillomavirus type 1Planta, 236
Li Liu, Jef Grainger, M. Cañizares, S. Angell, G. Lomonossoff (2004)
Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing.Virology, 323 1
Frank Sainsbury, Li Liu, G. Lomonossoff (2009)
Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants.Methods in molecular biology, 483
The pEAQ vectors are a series of plasmids designed to allow easy and quick production of recombinant proteins in plants. Their main feature is the use of the Cowpea Mosaic Virus hypertranslational “CPMV-HT” expression system, which provides high yields of recombinant protein through extremely high translational efficiency without the need for viral replication. Since their creation, the pEAQ vectors have been used to produce a wide variety of proteins in plants. Viral proteins and Virus-Like Particles (VLPs) have been of particular interest, but other types of proteins including active enzymes have also been expressed. While the pEAQ vectors have mostly been used in a transient expression context, through agroinfiltration of leaves, they have also been shown to be suitable for the production of stably transformed lines of both cell cultures and whole plants. This paper looks back on the genesis of the pEAQ vectors and reviews their use so far.
Plant Molecular Biology – Springer Journals
Published: Mar 12, 2013
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