264 Mammalian Genome 9, Brief Data Report Fig. 1. (a) CHRNA3: Segregation of the Cfol-polymorphism in a PiGMaP CHRNB4: SSCP analysis of four PiGMaP family members showing the family. The estimated fragment length is indicated beside the photo in bp, two different alleles. Genotypes are indicated below each lane. and the genotypes of the individuals are shown below the tracks. (b) product (15 pl) with 5 U discussions. This work is part of the PiGMaP international genetic mapping Cfol at 37 °C overnight results in frag- collaboration and was supported by grants from the German National Sci- ments shown in Fig. la. ence Foundation DFG and the Akademie fur Tiergesundheit. For CHRNB4, a diallelic single strand conformation polymor- phism (SSCP) was observed (Fig. lb). The PCR product was References mixed with a 2 x volume of formamide loading buffer. After being 1. Archibald AL, Haley CS, Brown JF, Couperwhite S, McQueen HA, heated to 95°C for 5 min, the samples were placed immediately on Nicholson D, Coppieters W, Van de Weghe A, Stratil A, Wintero AK, ice and separated on a 0.5 x MDE (FMC, Rockland) 0.6 x TBE gel Fredholm M, Larsen NJ, Nielsen VH, Milan D, Woloszyn
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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