ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 6, pp. 666–671. © Pleiades Publishing, Inc., 2010.
Original Russian Text © A.A. Konovalov, E.A. Moiseeva, N.P. Goncharov, E.Ya. Kondratenko, 2010, published in Genetika, 2010, Vol. 46, No. 6, pp. 758–763.
A linkage between stem characters and aromatic
metabolism enzymes has been observed in several
plant species [1–3]. Several enzymes with similar
functions are known to reduce aromatic aldehydes to
the corresponding alcohols: EC 184.108.40.206, EC 220.127.116.11,
EC 18.104.22.168, and EC 22.214.171.124. The enzymes cata
lyze the penultimate reaction of stem lignification and
produce aromatic alcohols (monolignols). Monolog
nols are used to produce lignin, which is a three
dimensional polymer that determines the main dura
bility characteristics of stems in vascular plants (along
with cellulose microfibrils) [4, 5].
Rye plants with fragile stems have been observed in
many studies [6–10]. The character was determined as
(brittle straw); the designation
(fragile) has been proposed for fragile stems and spikes
in . The mutant form used in our work was desig
Chromosome 5R harbors a gene(s) that determines
the growth habit. The growth habit is variously desig
(spring growth habit),
, the Latin for sum
mer). In rye, the character is controlled by the
) gene, which is in the long arm of chromosome 5R
[11, 12]. We used the symbols
(spring habit) and
The localization of the genes under study in the
arms of chromosome 5R is known; i.e., the
) genes are in the short arm, and the
) genes are in the long arm .
The order of the genes is unclear.
In common wheat, the
are in the short arms of the chromosomes of
homeology group 5, and the
are in the long arms of the same chro
mosomes, respectively [13, 14].
In this work, we studied the segregation for the
growth habit, straw fragility, and allelic variants of aro
matic alcohol dehydrogenase and shikimate dehydro
genase in the progenies of analyzing crosses to assess
the linkage of the characters, to establish the order of
the corresponding genes in chromosome 5R, and to
estimate the recombination coefficients.
MATERIALS AND METHODS
The phenotypes for the characters under study are
shown in Figs. 1–3. Fragilestraw winter rye (geno
) was obtained from the AllRussian Institute
of Plant Industry. The accession (k11511) was iso
lated from a winter variety by V.D. Kobylyanskii in
1969 and was examined by Surikov and Romanova .
The Order of the
Genes in Chromosome 5R
A. A. Konovalov, E. A. Moiseeva, N. P. Goncharov, and E. Ya. Kondratenko
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia;
Received April 23, 2009
—Segregation analysis was performed in the progenies obtained in analyzing crosses of hybrids of
spring and winter accessions of rye
L. and wild
(J. Presl) J. Presl). The test genes controlled the brittle stem (
), the allelic variants of aro
matic alcohol dehydrogenase (
) and shikimate dehydrogenase (
), and the growth habit (
linkage was observed in the inheritance of the brittle stem and the aromatic alcohol dehydrogenase and shiki
mate dehydrogenase alloenzymes. The order of genes was established to be
, and the genetic
distances were estimated to be
recombination coefficient between the
genes varied from 2.2 to 18.2%, averaging 5.3%. The
growth habit was inherited independently of the
Zymogram of the fast region of aromatic alcohol
dehydrogenase 1 (AADH1) in rye.
) heterogzygote FS, (
3, 4, 10
) homozygote SS, and (
) homozygote FF.