Arch Virol (1998) 143: 2461–2469
The nucleotide sequence of the 3
-terminal region of dasheen
mosaic virus (Caladium isolate) and expression of its coat protein
in Escherichia coli for antiserum production
R. H. Li
, F. W. Zettler, D. E. Purcifull, and E. Hiebert
Department of Plant Pathology, University of Florida, Gainesville, Florida, U.S.A.
Accepted June 26, 1998
Summary. A caladium isolate of dasheen mosaic virus (DsMV-Ch) was cloned
as cDNA from genomic RNA. The sequence of the 3
-terminal 3 158 nucleotides,
which consisted of the 3
-terminus of the NIa gene, the NIb gene, the coat protein
(CP) gene, and a 246-nucleotide non-coding region, was between 57–68% similar
at the nucleotide level and 72–82% similar at the amino acid level when compared
with other potyviruses. Phylogenetic analysis of aligned, selected potyviral CP
sequences indicate that DsMV-Ch is similar to DsMV isolates infecting taro and
closely related to the bean common mosaic virus subgroup in the genus Potyvirus.
A recombinant DsMV-Ch CP (∼39 kDa) expressed in E. coli was used as an
immunogen and the resulting antiserum reacted with DsMV and several other
potyviruses in Western blots and indirect ELISA.
Dasheen mosaic potyvirus (DsMV) is the most important viral pathogen of
cultivated aroid plants worldwide [22, 25]. It causes serious damage to the orna-
mentals, Caladium, Dieffenbachia, and Zantedeschia, and is ubiquitous in com-
mercial plantings of the tropical root crops, Colocasia and Xanthosoma spp. .
Likeotherpotyviruses,DsMVhasﬂexuous,ﬁlamentous particles about 750 nm in
length, which contain a positive sense, single-stranded RNA of Mr 3.2–3.4 × 10
[1, 12, 15]. DsMV is serologically related to certain other potyviruses [1, 12, 15].
We report here the cDNA cloning of a DsMV isolate (DsMV-Ch) from
Caladium hortulanum, and the nucleotide sequence of its 3
Present address: Plant Pathology Department, Auburn University, Auburn, AL, U.S.A.