Arch Virol (2000) 145: 651–657
The nucleotide sequence and genome organization of Japanese iris
necrotic ring virus, a new species in the genus Carmovirus
, T. Kanehira
, M. Shinohara
, S. Yamashita
, and T. Hibi
Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology,
The University of Tokyo, Tokyo, Japan
College of Bioresource Sciences, Nihon University,
Fujisawa, Kanagawa, Japan
Accepted September 23, 1999
Summary. The genome of Japanese iris necrotic ring virus (JINRV) consists of
a positive-sense ssRNA of 4014 nucleotides with six major open reading frames
(ORFs). A 5
-non-coding region of 31 nucleotides precedes the ﬁrst initiation
codon. Like Carnation mottle virus (CarMV), the 5
-proximal three ORFs encode
a 26 kDa protein (p26) and two readthrough proteins, i.e. an 85 kDa putative RNA
replicase (p85) and a 99 kDa protein (p99). The central ORF encodes a small
8 kDa protein (p8). The 3
-proximal ORF encodes a 38 kDa capsid protein (p38).
Another ORFencodinga 12 kDa protein (p12) overlapsthe p99 ORF.JINRV RNA
treated with bacterial alkaline phosphatase and tobacco acid pyrophosphatase
could not be ligated to an oligoribonucleotide using T4 RNA ligase, indicating
that the 5
end of the viral RNA is uncapped. The 3
end is not polyadenylated.
Comparison of the genomic organization and the predicted amino acid sequences
with those of other viruses conﬁrmed that JINRV should be classiﬁed as a member
of the genus Carmovirus, family Tombusviridae.
Japanese iris necrotic ring virus (JINRV), causing spindle-shaped necrotic rings
on the leaves of Japanese iris (Iris ensata Thunb.), was ﬁrst reported in Japan
. JINRV has extremely narrow natural and experimental host ranges limited
to a few species of Iridaceae, and is transmitted mechanically but not by aphids
or through the soil .
The nucleotide sequence presented in this paper has been deposited in DDBJ, EMBL
and GenBank nucleotide sequence databases under the accession number D86123.