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S Oh, VK Verselis, TA Bargiello (2008)
Charges dispersed over the permeation pathway determine the charge selectivity and conductance of a Cx32 chimeric hemichannelJ Physiol, 586
EB Trexler, MV Bennett, TA Bargiello, VK Verselis (1996)
Voltage gating and permeation in a gap junction hemichannelProc Natl Acad Sci USA, 93
VK Verselis, CS Ginter, TA Bargiello (1994)
Opposite voltage gating polarities of two closely related connexinsNature, 368
J Neijssen, C Herberts, JW Drijfhout, E Reits, L Janssen, J Neefjes (2005)
Cross-presentation by intercellular peptide transfer through gap junctionsNature, 434
S Maeda, S Nakagawa, M Suga, E Yamashita, A Oshima, Y Fujiyoshi, T Tsukihara (2009)
Structure of the connexin 26 gap junction channel at 3.5 A resolutionNature, 458
HA Sanchez, G Mese, M Srinivas, TW White, VK Verselis (2010)
Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40 V and G45E mutations that cause keratitis ichthyosis deafness syndromeJ Gen Physiol, 136
V Valiunas, YY Polosina, H Miller, IA Potapova, L Valiuniene, S Doronin, RT Mathias, RB Robinson, MR Rosen, IS Cohen, PR Brink (2005)
Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctionsJ Physiol, 568
M Srinivas, M Costa, Y Gao, A Fort, GI Fishman, DC Spray (1999)
Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cellsJ Physiol (Lond), 517
D Locke, S Bian, H Li, AL Harris (2009)
Post-translational modifications of connexin26 revealed by mass spectrometryBiochem J, 424
TW White, R Bruzzone, DA Goodenough, DL Paul (1992)
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70Mol Biol Cell, 3
AL Harris (2001)
Emerging issues of connexin channels: biophysics fills the gapQ Rev Biophys, 34
DC Spray, AL Harris, MV Bennett (1981)
Equilibrium properties of a voltage-dependent junctional conductanceJ Gen Physiol, 77
JJ Tong, L Ebihara (2006)
Structural determinants for the differences in voltage gating of chicken Cx56 and Cx45.6 gap-junctional hemichannelsBiophys J, 91
MV Bennett, LC Barrio, TA Bargiello, DC Spray, E Hertzberg, JC Saez (1991)
Gap junctions: new tools, new answers, new questionsNeuron, 6
FF Bukauskas, A Bukauskiene, MV Bennett, VK Verselis (2001)
Gating properties of gap junction channels assembled from connexin43 and connexin43 fused with green fluorescent proteinBiophys J, 81
AL Harris, DC Spray, MV Bennett (1981)
Kinetic properties of a voltage-dependent junctional conductanceJ Gen Physiol, 77
RD Veenstra, HZ Wang, DA Beblo, MG Chilton, AL Harris, EC Beyer, PR Brink (1995)
Selectivity of connexin-specific gap junctions does not correlate with channel conductanceCirc Res, 77
GS Goldberg, V Valiunas, PR Brink (2004)
Selective permeability of gap junction channelsBiochim Biophys Acta, 1662
VK Verselis, R Veenstra (2000)
Gap junctions
FF Bukauskas, VK Verselis (2004)
Gap junction channel gatingBiochim Biophys Acta, 1662
XW Zhou, A Pfahnl, R Werner, A Hudder, A Llanes, A Luebke, G Dahl (1997)
Identification of a pore lining segment in gap junction hemichannelsBiophys J, 72
T Kwon, AL Harris, A Rossi, TA Bargiello (2011)
Molecular dynamics simulations of the Cx26 hemichannel: evaluation of structural models with Brownian dynamicsJ Gen Physiol, 138
VK Verselis (2009)
Connexins: a guide
JR Lee, AM Derosa, TW White (2009)
Connexin mutations causing skin disease and deafness increase hemichannel activity and cell death when expressed in Xenopus oocytesJ Invest Dermatol, 129
K Willecke, J Eiberger, J Degen, D Eckardt, A Romualdi, M Guldenagel, U Deutsch, G Sohl (2002)
Structural and functional diversity of connexin genes in the mouse and human genomeBiol Chem, 383
PE Purnick, S Oh, CK Abrams, VK Verselis, TA Bargiello (2000)
Reversal of the gating polarity of gap junctions by negative charge substitutions in the N-terminus of connexin 32Biophys J, 79
HS Duffy, M Delmar, DC Spray (2002)
Formation of the gap junction nexus: binding partners for connexinsJ Physiol Paris, 96
X Hu, M Ma, G Dahl (2006)
Conductance of connexin hemichannels segregates with the first transmembrane segmentBiophys J, 90
AR Janecke, HC Hennies, B Gunther, G Gansl, J Smolle, EM Messmer, G Utermann, O Rittinger (2005)
GJB2 mutations in keratitis-ichthyosis-deafness syndrome including its fatal formAm J Med Genet A, 133
VK Verselis, MP Trelles, C Rubinos, TA Bargiello, M Srinivas (2009)
Loop gating of connexin hemichannels involves movement of pore-lining residues in the first extracellular loop domainJ Biol Chem, 284
PA Weber, HC Chang, KE Spaeth, JM Nitsche, BJ Nicholson (2004)
The permeability of gap junction channels to probes of different size is dependent on connexin composition and permeant-pore affinitiesBiophys J, 87
AP Moreno, M Chanson, S Elenes, J Anumonwo, I Scerri, H Gu, SM Taffet, M Delmar (2002)
Role of the carboxyl terminal of connexin43 in transjunctional fast voltage gatingCirc Res, 90
A Karlin, MH Akabas (1998)
Substituted-cysteine accessibility methodMethods Enzymol, 293
JJ Tong, X Liu, L Dong, L Ebihara (2004)
Exchange of gating properties between rat cx46 and chicken cx45.6Biophys J, 87
Y Ri, JA Ballesteros, CK Abrams, S Oh, VK Verselis, H Weinstein, TA Bargiello (1999)
The role of a conserved proline residue in mediating conformational changes associated with voltage gating of Cx32 gap junctionsBiophys J, 76
EB Trexler, FF Bukauskas, J Kronengold, TA Bargiello, VK Verselis (2000)
The first extracellular loop domain is a major determinant of charge selectivity in connexin46 channelsBiophys J, 79
M Srinivas, J Kronengold, FF Bukauskas, TA Bargiello, VK Verselis (2005)
Correlative studies of gating in Cx46 and Cx50 hemichannels and gap junction channelsBiophys J, 88
J Kronengold, EB Trexler, FF Bukauskas, TA Bargiello, VK Verselis (2003)
Single-channel SCAM identifies pore-lining residues in the first extracellular loop and first transmembrane domains of Cx46 hemichannelsJ Gen Physiol, 122
WH Evans, E Vuyst, L Leybaert (2006)
The gap junction cellular internet: connexin hemichannels enter the signalling limelightBiochem J, 397
J Kronengold, EB Trexler, FF Bukauskas, TA Bargiello, VK Verselis (2003)
Pore-lining residues identified by single channel SCAM studies in Cx46 hemichannelsCell Commun Adhes, 10
IM Skerrett, J Aronowitz, JH Shin, G Cymes, E Kasperek, FL Cao, BJ Nicholson (2002)
Identification of amino acid residues lining the pore of a gap junction channelJ Cell Biol, 159
Connexins form channels with large aqueous pores that mediate fluxes of inorganic ions and biological signaling molecules. Studies aimed at identifying the connexin pore now include a crystal structure that provides details of putative pore-lining residues that need to be verified using independent biophysical approaches. Here we extended our initial cysteine-scanning studies of the TM1/E1 region of Cx46 hemichannels to include TM2 and TM3 transmembrane segments. No evidence of reactivity was observed in either TM2 or TM3 probed with small or large thiol-modifying reagents. Several identified pore residues in E1 of Cx46 have been verified in different Cx isoforms. Use of variety of thiol reagents indicates that the connexin hemichannel pore is large and flexible enough, at least in the extracellular part of the pore funnel, to accommodate uncommonly large side chains. We also find that that gating characteristics are largely determined by the same domains that constitute the pore. These data indicate that biophysical and structural studies are converging towards a view that the N-terminal half of the Cx protein contains the principal components of the pore and gating elements, with NT, TM1 and E1 forming the pore funnel.
The Journal of Membrane Biology – Springer Journals
Published: Jul 24, 2012
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