Brief Data Reports Mammalian Genome 8,376-384 (1997). 9 Springer-Verlag New York Inc. 1997 Mapping of the eukaryotic initiation factor elF-1A gene, Eifla, to mouse Chromosome 12D-E by FISH N. Kikyo, M. Tada, T. Tada, M.A. Surani Wellcome/CRC Institute of Cancer and Developmental Biology, and Physiological Laboratory, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK Received: 31 October 1996 / Accepted: 12 January 1997 Species: Mouse Locus name: Eukaryotic initiation factor 1A (eIF-1A) Locus symbol: Eifla Map position: mouse Chromosome (Chr) 12 band D-E Method of mapping: FISH. The cDNA probe was labeled with biotin-16-dUTP, and the signal was detected by streptavidin-FITC (BRL) and biotinylated anti-avidin D (Vector) as described . Chromosomes were counterstained with propidium iodide and G- banded with DAPI. Database deposit information: GenBank accession number for sequence is U28419. Mouse Genome Database accession number for mapping is MGD-INEX-33. Molecular reagents used for mapping: D52, a 1.9-kb cDNA probe encoding Eifla was used in FISH. Previously identified homologs: Saccharomyces cerevisiae homo- log, TIFll , is at YMR260C. Human, rabbit, and wheat ho- mologs  have not been mapped yet. Fig. 1. FISH analysis of the Eifla gene. (A) Arrows indicate signals on Chr 12. (B) G-banding
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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