The mouse neuronal apoptosis inhibitory protein gene maps to a conserved syntenic region of mouse chromosome 13

The mouse neuronal apoptosis inhibitory protein gene maps to a conserved syntenic region of mouse... Brief Data Reports Mammalian Genome 8,222-227 (1997). 9 Springer-Verlag New York Inc. 1997 The mouse neuronal apoptosis inhibitory Jackson BSS protein gene maps to a conserved syntenic region of mouse Chromosome 13 D13Bir16 i D13Mit9 D13Bir17, D13Mit27 Christine J. DiDonato, 1 Joseph H. Nadeau, 2 D13Mit105, Dhfr DI3Hun11 Louise R. Simard 1 D13Mit104, D13Mit145 Fig. 1. Map showing part of Chr 13 D13Bir19 from The Jackson BSS backcross panel. The map is depicted with the D13Lsd1, D13Mit3 7 1Services de Grnrtique Mrdicale et de Neurologie, Centre de centromere toward the top. A 3-cM Recherche, Hrpital Ste-Justine, Qurbec, Canada H3T 1C5 scale bar is shown to the fight of 2Department of Genetics, Case Western Reserve University, the figure. Loci mapping to the Cleveland, Ohio 33106-4955, USA same position are listed in alphabetical order. Details are available from MGD. Raw data Received: 14 November 1996 / Accepted: 19 November 1996 from The Jackson Laboratory were obtained from the World Wide Web Etla address: http:/www, Species: Mouse D13Bir20, D13M~5 resources/documents/cmdata. Locus name: Neuronal apoptosis inhibitory protein "Locus symbol: Naip Map position: D13Mit104-(1.06 + 1.06)-D13Bir19-(2.13 + 1.49)-D13Lsd1, D13Mit37-(11.70 + 3.32)-Efta-(1.06 + 1.06)- atrophy (SMA) indicated that deletions of the telomeric survival D13Mit35 motor neuron gene and the intact NAIP gene were responsible for Method of mapping: Haplotype analysis of 94 progeny from The SMA [2,3]. However, the telomeric survivai motor neuron gene Jackson Laboratory BSS interspecific backcross panel [(C57BL/ appears to be the critical gene because causative point mutations 6JEi x SPRET/Ei)F l x SPRET/Ei] [1] with the polymorphic have been identified [2,4]. marker D13Lsdl. Since NAIP's role in SMA etiology cannot be adequately ad- Database deposit information and accession number: MGD- dressed in humans, we have begun to characterize the SMA region CREX-705 in the mouse. As a first step, we report the mapping of the Naip Molecular reagents: D13Lsd1 was isolated from a 129/SvJ geno- gene to the conserved syntenic region of mouse Chr 13 (Fig. 1) mic phage clone that contained coding sequence corresponding to with D13Lsd1. This marker may prove useful in refining the po- exons 5 and 6 of the human NAIP eDNA. Dl3Lsdl primers: For sition of other genes within this region such as Lgnl [5,6]. 5'-ACA TAC ATT CAC CCA CAC TC; Rev 5'-CCA gTT TTC CTg gCA AgA CA. Genetic mapping: D13Lsd1 PCRs contained 25 ng of genomic Acknowledgments: We thank Mary Barter and Lucy Rowe at The Jackson DNA, 2.5 mM MgC12, 4 IxM of each primer, 300 IXM of dATP, Laboratory for analysis of the interspecific backcross data. This work was dGTP and dTTP, 150 IXM dCTP, 44 nM of [32P]-dCTP, lx supplied supported by a grant from the Medical Research Council (MRC) of reaction buffer and 1 U of Taq DNA polymerase (Gibco BRL) in Canada. L.R. Simard is a Fonds de la Recherche en Sant6 du Qurbec Scholar. a final volume of 50 ixl. Cycling conditions consisted of an initial 3-min denaturation step at 94~ followed by 35 cycles of 94~ for 30 s, 50~ for 30 s, and 72~ for 30 s. D13Lsd1 amplified a References product of 114 bp, 106 bp, 130 bp, and 124 bp with 129/SvJ, 1. Rowe LB, Nadeau JH, Turner R, Frankel WN, Letts VA, Eppig JT, Ko C57BL/6JEi, SPRET/Ei and A/J genomic DNA respectively. Am- MS, Thurston S J, Birkenmeier EH (1994) Mamm Genome 5, 253-274 plification products were run in 6% denaturing polyacrylamide 2. Lefebvre S, B~rglen L, Reboullet S, Clermont O, Burlet P, Violett L, gels. Benichou B, Cruaud C, Millasseau P, Zeviani M, Le Paslier D, Frezal Previously identified homolog: The human neuronal apoptosis F, Cohen D, Weissenbach J, Munnich A, Melki J (1995) Cell 80, 155- inhibitory protein (NAIP) gene has been assigned to 5ql 1.2-ql 3.3 [2,3]. 3. Roy N, Mahadevan MS, McLean M, Shutler G, Yaraghi Z, Farahani R, Discussion: The NAIP gene has been mapped to a complex in- Baird S, Besner-Johnston A, Lefebrve C, Xiaolin K, Salih M, Aubry H, verted duplication on human Chromosome (Chr) 5q 11.2-ql 3.3 [2]. Tamai K, Guan XP, Ioannou P, Crawford TO, de Jong P, Surh L, lkeda JE, Korneluk RG, MacKenzie AE (1995) Cell 80, 167-178 Duplications in this region have resulted in multiple copies of 4. Bussaglia E, Clermont O, Tizzano E, Lefebvre S, Burglen L, Cruaud C, NAIP and other genes, but only one NAIP gene is intact [3]. Urtizberea JA, Colomer J, Munnich A, Baiget M, Melki J (1995) Na- Analysis of DNAs from patients with proximal spinal muscular ture Genet 11,335-337 5. Beckers MC, Yoshida S, Morgan K, Gros P (1995) Mamm Genome 6, 540-545 6. Dietrich WF, Damron DM, Isberg RR, Lander ES, Swanson MS (1995) Correspondence to: L. R. Simard Genomics 26, 443-450 Mammalian Genome Springer Journals

The mouse neuronal apoptosis inhibitory protein gene maps to a conserved syntenic region of mouse chromosome 13

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Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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