Brief Data Reports -Ma a aft n G^^ Po a„^g „o^, (0,^. Mammalian Genome 9, 339-342 (1998). © Springer-Verlag New York Inc. 1998 The mouse homologs of human GIF, DDB1, and CFL1 genes are located on Chromosome 19 Marie Fernandes,' Christophe Poirier 2, Frangoise Lespinasse l , Georges F. Carle' 'CNRS/UNSA UMR 6549, Faculte de Medecine, Avenue de Valombrose, 06107 Nice cedex 2, France 2Unite de Genetique des Mammiferes, 25 rue du docteur Roux, Institut Pasteur, 75724 Paris cedex 15, France Received: 18 December 1997 / Accepted: 19 December 1997 Species: Mouse Locus name: Mouse p127 subunit of damage-specific DNA bind- ing protein, mouse gastric intrinsic factor, and mouse cofilin 1 (non-muscle isoform). Locus symbol: Ddbl, Gif, cfll. Map position: Mouse Chromosome 19 Methods of mapping: Fluorescent in situ hybridization (FISH) on mouse SV22-CD cell line metaphase chromosomes . Mouse x hamster somatic cell hybrids analysis. Molecular reagents: The human 4.2-kb DDB 1 cDNA [21 was labeled with biotinylated-14-dUTP and nick-translation kit GIBCO BRL (No. 8247 SA), hybridized (200 ng per slide) in the presence of 10 p.g of mouse Cotl DNA (Life Technologies- Fig. 1. Fluorescent in situ hybridization of the human DDB1 probe on GIBCO BRL, Cergy Pomtoise, France), and revealed by avidin- mouse SV22CD cell line metaphase chromosomes . The probe was labeled with biotinylated-14-dUTP and a nick-translation kit GIBCO BRL FITC (Vector Laboratories) as previously described . Meta- (n° 8247 SA) and hybridized as previously described . Metaphase phase spreads were analyzed under an Axiophot (Zeiss) micro- spreads were analyzed under an Axiophot (Zeiss) microscope. The arrow scope (Fig. 1). For we have selected a pair of primers allowing Gif indicates the signals position on MMU19. amplification of a 120-bp fragment of exon 1 (GeneBank Acc # L24192) on mouse DNA. Gif-a: 5' aag tgc aca cag gag aag gta gag Gif-z: 5' cac agc cca gag aac act tag ga. A pair of primers for Cfll recipient of a fellowship from Association pour la Recherche contre le was selected on mouse EST sequence (GeneBank Acc #W09648) Cancer, and C. Poirier is a recipient of a fellowship from Association and allowed amplification of a 195-bp fragment on mouse DNA. Francaise contre les Myopathies. This work was supported by grants from COI-a: 5' caa cct atg aga cca agg aga gca Cfll-z: 5' ctt gac ctt cct the Association pour la Recherche contre le Cancer. cgt agc agt tag PCR was performed as previously described. Previously identified homologs: Human homologs [2, 4, 5]. CFL1 homologs: Gallus domesticus destrin gene (SWISSPROT Acc References #P18359). GIF homolog: rat Gif gene (GenBank Acc #D45200, 1. Baron B, Met6zeau P, Kelly F, Bernheim A, Berger R, Guenet J-L, J03577). Golberg ME (1984) Exp Cell Res 152, 220-340 Discussion: FISH experiment performed with the human DDB1 2. Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S (1995) cDNA has shown a specific and recurrent signal on mouse Chr 19 Genomics 29, 62-69 only, near the centromere. We thus concluded that the mouse 3. Fernandes M, Poirier C, Lassam NJ, Larsson C, Guenet J-L, Gaudray P, ortholog for DDB1 is located on the pericentromeric region of Carle GF (1997) Mamm Genome 8, 513-515 mouse Chr 19 (Fig. 1). 4. Gillett GT, Fox MF, Rowe PS, Casimir CM, Povey S (1996) Ann Hum For Gif and Cfll genes, PCR was carried out on a somatic cell Genet 60, 201-211 hybrids panel  and allowed the mapping of these two genes on Hewitt JE, Gordon MM, Taggart RT, Mohandas TK, Alpers DH (1991) mouse Chr 19. These three genes might be used as landmarks in Genomics 10, 432-440 comparative mapping studies  and as candidate genes for mouse 6. O'Brien Si, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland mutant loci located on mouse Chr 19 . Our finding validates the NG (1993) Nat Genet 3, 103-112 approach relying on searching genes in syntenic regions known to Poirier C, Guenet J-L (1997) Mamm Genome 7, 305-312 be conserved between human and mouse and supports the high Shows TB, Alders M, Bennett S, Burbee D, Cartwright P, Chan- degree of homology between human 11g13 and mouse Chr 19 . drasekharappa S, Cooper P, Courseaux A, Davies C, Devignes MD, Devilee P, Elliott R, Evans G, Fantes J, Gamer H, Gaudray P, Gerhard Acknowledgments: We thank Stuart Linn and Anne F. Nichols (University DS, Gessler M, Higgins M, Hummerich H, James M, Lagercrantz J, Litt of California, Berkeley) for the DDB 1 cDNA clone. M. Fernandes is a M, Little P, Zabel B, et al. (1996) Cytogenet Cell Genet 74, 1-56 9. Williamson P, Holt S, Townsend S, and Boyd Y (1995) Mamm Genome Correspondence to: G.F. Carle 6, 429-432
Mammalian Genome – Springer Journals
Published: Mar 20, 2009
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