Plant Mol Biol (2017) 94:149–165 DOI 10.1007/s11103-017-0601-0 The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes 1 1 1 1 1 Haifeng Xu · Nan Wang · Jingxuan Liu · Changzhi Qu · Yicheng Wang · 1 2 1 1 1 Shenghui Jiang · Ninglin Lu · Deyun Wang · Zongying Zhang · Xuesen Chen Received: 16 December 2016 / Accepted: 27 February 2017 / Published online: 12 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract no inhibitory effect on anthocyanin. The yeast one-hybrid Key message MdMYB16 forms homodimers and assay showed that MdMYB16 and LESMdMYB16 inter- directly inhibits anthocyanin synthesis via its C-termi- acted the promoters of MdANS and MdUFGT, respectively. nal EAR repressor. It weakened the inhibitory effect of Yeast two-hybrid, pull-down, and bimolecular fluorescence MdMYB16 on anthocyanin synthesis when overexpress- complementation assays showed that MdMYB16 formed ing MdbHLH33 in callus overexpressing MdMYB16. homodimers and interacted with MdbHLH33, however, MdMYB16 could interact with MdbHLH33. the LBSMdMYB16 could not interact with MdbHLH33. Abstract Anthocyanins are strong antioxidants that play a We overexpressed MdbHLH33 in callus overexpressing key role in the prevention of cardiovascular disease, can- MdMYB16 and found that it weakened the inhibitory effect cer, and diabetes. The germplasm of Malus sieversii f. of
Plant Molecular Biology – Springer Journals
Published: Mar 12, 2017
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