The leptin receptor gene (LEPR) maps to porcine chromosome 6

The leptin receptor gene (LEPR) maps to porcine chromosome 6 226 Mammalian Genome 8, Brief Data Reports Acknowledgments: We thank The Jackson Laboratory for providing the backcross DNA and are especially grateful to Lucy Rowe for her help in generating the haplotype and mapping data. Supported by NIH grant GM- References 1. Manly K (1993) Mamm Genome 4, 303-313 2. Rowe LB, Nadeau JH, Turner R, Frankel WN, Letts VA, Eppig JT, Ko MS, Thurston SJ, Birkenmeier EH (1994) Mamm Genome 5,253-274 3. Amor-Gueret M, Levi-Strauss M (1990) Nucleic Acids Res 18, 1646 4. Naga Y, Srinivasan M, Platero JS, Svendrowski M, Waheed A, Sly WS (1994) Proc Natl Acad Sci USA 91, 10330-10334 5. Nagao Y, Batanian JR, Clements MF, Sly WS (1996) Genomics 28, Chromosome 6 477-484 Fig. l. Diagram representing the presence of fragments of porcine Chr 6 6. Dodgson SJ (1991) In The Carbonic Anhydrases: Cellular Physiology in each hybrid clone. The chromosome fragments are shown as solid bars and Molecular Genetics, SJ Dodgson, RE Tashian, G Gros, ND Carter, spanning the length of the fragment. The presence of various Chr 6 frag- eds. (Plenum Press, New York) pp 297-306 ments enables the definition of regions named by a capital letter and 7. Hewett-Emmett D, Tashian RE (1996) Mol Phylogenet Evol 5, 50-75 alternatively represented in gray or white. Regions I and K were retained 8. Beechey C, Tweedie S, Spurt N, Ball S, Peters J, Edwards Y (1989) by the same hybrids and thus can not be distinguished. Positive hybrids for Genomics 4, 672-696 LEPR were 9, 10, 14, 18, and 26, indicating that LEPR maps to either 9. Kelly C, Nogradi A, Walker R, Caddy K, Peters J, Carter N (1994) Region I or Region K. Biochem Soc Trans 22, 359S 10. Ceci JD (1996) Mamm Genome 6 Suppl, $151-S169 11. Debry RE, Seldin MF (1996) Genomics 33, 337-351 shown to map to within a 5.1-cM interval of mouse Chr. 4 which contains the db locus [7]. This report was followed by two studies providing evidence that db was the gene encoding OB-R [12,13]. The leptin receptor gene (LEPR) maps to A recent report by Chua and associates [8] has confirmed that db, fa and OB-R are the same gene. The mouse leptin receptor gene porcine Chromosome 6 has now been assigned the symbol, Lepr, which replaces the pre- viously used symbols OB-R and Obr. Mapping of the human leptin Catherine W. Ernst, 1 Paul A. Kapke, 1 receptor gene (LEPR) has also recently been reported [4-6]. Martine Yerle, 2 Max F. Rothschild 1 The assignment of LEPR to porcine Chr. 6q3.3-3.5 is in agree- ment with the previous assignment of this gene to human Chr 1 p32 1Department of Animal Science, 225 Kildee Hall, Iowa State University, since bidirectional chromosome painting analysis has demon- Ames, Iowa 50011-3150, USA strated correspondence between these human and porcine chromo- 2Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan, France somal segments [14]. This information extends the comparative mapping information for LEPR since it has also been mapped to Received: 2 October 1996 / Accepted: 19 November 1996 homologous segments of mouse Chr 4 and rat Chr 5. Species: Pig Locus name: Leptin receptor Acknowledgments: This work was supported in part by the Iowa Agricul- ture and Home Economics Experiment Station, Ames, Journal paper No. Locus symbol: LEPR J-17087, Project No. 3148. This work is in part supported by a grant from Map position: 6q3.3-q3.5 Dalgety Food Technology Center and PIC. Method of mapping: Somatic cell hybrid panel Database deposit information: GenBank accession number References U72070 1. Yerle M, Echard G, Robic A, Mairal A, Dubut-Fontana C, Riquet J, Molecular reagents: Oligonucleotide primers designed from hu- Pinton P, Milan D, Lahbib-Mansais Y, Gellin J (1996) Cytogenet Cell man cDNA sequence (GenBank accession number U43168) were Genet 73, 194-202 used to amplify a 380-bp fragment of the porcine LEPR gene. 2. Robic A, Riquet J, Yerle M, Milan D, Lahbib-Mansais Y, Dubut- Fontana C, Gellin J (1996) Mamm Genome 7, 438-445 Sequence similarity between human cDNA and porcine exonic 3. Chevalet C, Gouzy J, SanCristobal-Gaudy M (1996) CABIOS, in press sequences was 93.7%. Primer sequences were: forward primer, 4. Chung WK, Powerkehoe L, Chua M, Leibel RI (1996) Genome Res 6, 5'-CCA AAC CTC GAG GAA AGT TTA CC-3'; and reverse 431-438 primer, 5'-AGG CTG CTC CTA TGA TAC CTC AA-3'. Analysis 5. Cioffi JA, Shafer AW, Zupancic TJ, Smith-Gbur J, Mikhail A, Platika of 27 porcine-rodent somatic cell hybrids [1,2] allowed regional D, Snodgrass HR (1996) Nature Med 2, 585-589 assignment of LEPR to porcine Chromosome (Chr) 6q3.3-3.5 with 6. Winick JD, Stoffel M, Friedman JM (1996) Genomics 36, 221-222 100% concordancy [3]. Positive LEPR signals were obtained for 7. Tartaglia LA, Dembski M, Weng X, Deng N, Culpepper J, Devos R, hybrids 9, 10, 14, 18, and 26 which are common only to regions I Richards GJ, Campfield LA, Clark FT, Deeds J, Muir C, Sanker S, Moriarty A, Moore KJ, Smutko JS, Mays GG, Woolf EA, Monroe CA, and K of Chr 6 (Fig. 1). Tepper RI (1995) Cell 83, 1263-1271 Previously identified homologs: Human LEPR has been localized 8. Chua SC Jr, Chung WK, Wu-Peng XS, Zhang Y, Liu S-M, Tartaglia Lepr maps to Chr 4 [7,8], and rat Lepr to Chr lp32 [4-6], mouse L, Leibel RL (1996) Science 271,994-996 maps to Chr 5 [8,9]. 9. Truett GE, Jacob HJ, Miller J, Drouin G, Bahary N, Smoller JW, Discussion: The murine autosomal recessive mutations obese (ob), Lander ES, Leibel RL (1995) Mamm Genome 6, 25-30 diabetes (db), and fatty (fa) were first reported in the 1960s [re- 10. Bray GA, York DA (1978) Physiol Rev 59, 719-809 viewed in 10]. The phenotypes of animals homozygous for these 11. Zhang Y, Proenca P, Maffei M, Barone M, Leopold L, Friedman JM mutations include severe, early-onset obesity, insulin resistance, (1994) Nature 372, 425-432 and susceptibility to diabetes. The ob gene has recently been 12. Chen H, Charlat O, Tartaglia LA, Woolf EA, Weng X, Ellis SJ, Lakey cloned in human and mouse and its protein product identified as ND, Culpepper J, Moore KJ, Breitbart RE, Duyk GM, Tepper RI, Morgenstem JP (1996) Cell 84, 491-495 leptin [11]. Subsequent research led to the identification of a re- 13. Lee G-H, Proenca R, Montez JM, Carroll KM, Darvishzadeh JG, Lee ceptor for leptin in mice (OB-R) [7]. The gene for OB-R was JI, Friedman JM (1996) Nature 379, 632-635 14. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat Correspondence to: M.F. Rothschild G, Gellin J (1996) Genomics 36, 252-262 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

The leptin receptor gene (LEPR) maps to porcine chromosome 6

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226 Mammalian Genome 8, Brief Data Reports Acknowledgments: We thank The Jackson Laboratory for providing the backcross DNA and are especially grateful to Lucy Rowe for her help in generating the haplotype and mapping data. Supported by NIH grant GM- References 1. Manly K (1993) Mamm Genome 4, 303-313 2. Rowe LB, Nadeau JH, Turner R, Frankel WN, Letts VA, Eppig JT, Ko MS, Thurston SJ, Birkenmeier EH (1994) Mamm Genome 5,253-274 3. Amor-Gueret M, Levi-Strauss M (1990) Nucleic Acids Res 18, 1646 4. Naga Y, Srinivasan M, Platero JS, Svendrowski M, Waheed A, Sly WS (1994) Proc Natl Acad Sci USA 91, 10330-10334 5. Nagao Y, Batanian JR, Clements MF, Sly WS (1996) Genomics 28, Chromosome 6 477-484 Fig. l. Diagram representing the presence of fragments of porcine Chr 6 6. Dodgson SJ (1991) In The Carbonic Anhydrases: Cellular Physiology in each hybrid clone. The chromosome fragments are shown as solid bars and Molecular Genetics, SJ Dodgson, RE Tashian, G Gros, ND Carter, spanning the length of the fragment. The presence of various Chr 6 frag- eds. (Plenum Press, New York) pp 297-306 ments enables the definition of regions named by a capital letter and 7. Hewett-Emmett D, Tashian RE (1996) Mol Phylogenet Evol 5, 50-75 alternatively represented in gray or white. Regions I and K were retained 8. Beechey C, Tweedie S, Spurt N, Ball S, Peters J, Edwards Y (1989) by the same hybrids and thus can not be distinguished. Positive hybrids for Genomics 4, 672-696 LEPR were 9, 10, 14, 18, and 26, indicating that LEPR maps to either 9. Kelly C, Nogradi A, Walker R, Caddy K, Peters J, Carter N (1994) Region I or Region K. Biochem Soc Trans 22, 359S 10. Ceci JD (1996) Mamm Genome 6 Suppl, $151-S169 11. Debry RE, Seldin MF (1996) Genomics 33, 337-351 shown to map to within a 5.1-cM interval of mouse Chr. 4 which contains the db locus [7]. This report was followed by two studies providing evidence that db was the gene encoding OB-R [12,13]. The leptin receptor gene (LEPR) maps to A recent report by Chua and associates [8] has confirmed that db, fa and OB-R are the same gene. The mouse leptin receptor gene porcine Chromosome 6 has now been assigned the symbol, Lepr, which replaces the pre- viously used symbols OB-R and Obr. Mapping of the human leptin Catherine W. Ernst, 1 Paul A. Kapke, 1 receptor gene (LEPR) has also recently been reported [4-6]. Martine Yerle, 2 Max F. Rothschild 1 The assignment of LEPR to porcine Chr. 6q3.3-3.5 is in agree- ment with the previous assignment of this gene to human Chr 1 p32 1Department of Animal Science, 225 Kildee Hall, Iowa State University, since bidirectional chromosome painting analysis has demon- Ames, Iowa 50011-3150, USA strated correspondence between these human and porcine chromo- 2Laboratoire de Genetique Cellulaire, INRA, Castanet-Tolosan, France somal segments [14]. This information extends the comparative mapping information for LEPR since it has also been mapped to Received: 2 October 1996 / Accepted: 19 November 1996 homologous segments of mouse Chr 4 and rat Chr 5. Species: Pig Locus name: Leptin receptor Acknowledgments: This work was supported in part by the Iowa Agricul- ture and Home Economics Experiment Station, Ames, Journal paper No. Locus symbol: LEPR J-17087, Project No. 3148. This work is in part supported by a grant from Map position: 6q3.3-q3.5 Dalgety Food Technology Center and PIC. Method of mapping: Somatic cell hybrid panel Database deposit information: GenBank accession number References U72070 1. Yerle M, Echard G, Robic A, Mairal A, Dubut-Fontana C, Riquet J, Molecular reagents: Oligonucleotide primers designed from hu- Pinton P, Milan D, Lahbib-Mansais Y, Gellin J (1996) Cytogenet Cell man cDNA sequence (GenBank accession number U43168) were Genet 73, 194-202 used to amplify a 380-bp fragment of the porcine LEPR gene. 2. Robic A, Riquet J, Yerle M, Milan D, Lahbib-Mansais Y, Dubut- Fontana C, Gellin J (1996) Mamm Genome 7, 438-445 Sequence similarity between human cDNA and porcine exonic 3. Chevalet C, Gouzy J, SanCristobal-Gaudy M (1996) CABIOS, in press sequences was 93.7%. Primer sequences were: forward primer, 4. Chung WK, Powerkehoe L, Chua M, Leibel RI (1996) Genome Res 6, 5'-CCA AAC CTC GAG GAA AGT TTA CC-3'; and reverse 431-438 primer, 5'-AGG CTG CTC CTA TGA TAC CTC AA-3'. Analysis 5. Cioffi JA, Shafer AW, Zupancic TJ, Smith-Gbur J, Mikhail A, Platika of 27 porcine-rodent somatic cell hybrids [1,2] allowed regional D, Snodgrass HR (1996) Nature Med 2, 585-589 assignment of LEPR to porcine Chromosome (Chr) 6q3.3-3.5 with 6. Winick JD, Stoffel M, Friedman JM (1996) Genomics 36, 221-222 100% concordancy [3]. Positive LEPR signals were obtained for 7. Tartaglia LA, Dembski M, Weng X, Deng N, Culpepper J, Devos R, hybrids 9, 10, 14, 18, and 26 which are common only to regions I Richards GJ, Campfield LA, Clark FT, Deeds J, Muir C, Sanker S, Moriarty A, Moore KJ, Smutko JS, Mays GG, Woolf EA, Monroe CA, and K of Chr 6 (Fig. 1). Tepper RI (1995) Cell 83, 1263-1271 Previously identified homologs: Human LEPR has been localized 8. Chua SC Jr, Chung WK, Wu-Peng XS, Zhang Y, Liu S-M, Tartaglia Lepr maps to Chr 4 [7,8], and rat Lepr to Chr lp32 [4-6], mouse L, Leibel RL (1996) Science 271,994-996 maps to Chr 5 [8,9]. 9. Truett GE, Jacob HJ, Miller J, Drouin G, Bahary N, Smoller JW, Discussion: The murine autosomal recessive mutations obese (ob), Lander ES, Leibel RL (1995) Mamm Genome 6, 25-30 diabetes (db), and fatty (fa) were first reported in the 1960s [re- 10. Bray GA, York DA (1978) Physiol Rev 59, 719-809 viewed in 10]. The phenotypes of animals homozygous for these 11. Zhang Y, Proenca P, Maffei M, Barone M, Leopold L, Friedman JM mutations include severe, early-onset obesity, insulin resistance, (1994) Nature 372, 425-432 and susceptibility to diabetes. The ob gene has recently been 12. Chen H, Charlat O, Tartaglia LA, Woolf EA, Weng X, Ellis SJ, Lakey cloned in human and mouse and its protein product identified as ND, Culpepper J, Moore KJ, Breitbart RE, Duyk GM, Tepper RI, Morgenstem JP (1996) Cell 84, 491-495 leptin [11]. Subsequent research led to the identification of a re- 13. Lee G-H, Proenca R, Montez JM, Carroll KM, Darvishzadeh JG, Lee ceptor for leptin in mice (OB-R) [7]. The gene for OB-R was JI, Friedman JM (1996) Nature 379, 632-635 14. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat Correspondence to: M.F. Rothschild G, Gellin J (1996) Genomics 36, 252-262

Journal

Mammalian GenomeSpringer Journals

Published: Mar 23, 2009

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