The Involvement of the Chloroplast Zeatin-Binding Protein in Hormone-Dependent Transcriptional Control of the Chloroplast Genome

The Involvement of the Chloroplast Zeatin-Binding Protein in Hormone-Dependent Transcriptional... Using a multistep system for protein purification, including affinity chromatography on zeatin riboside–Sepharose, we isolated a zeatin-binding protein from the chloroplasts of the first leaf of ten-day-old barley seedlings (ZBPChl). In combination with trans-zeatin, this protein enhanced the RNA synthesis in the chloroplast lysate from the same leaves. A hormonal specificity of ZBPChl-induced transcriptional stimulation was demonstrated. The protein activated the chloroplast but not nuclear genome. Age-dependent changes in the ZBPChl functional activity and in the response of the chloroplast transcription system to this protein and trans-zeatin were observed. In the chloroplast lysate from aging first leaves of 14-day-old barley plants, ZBPChl from the leaves of 3-, 10-, and 14-day-old barley plants activated transcription in the absence of the hormone, and trans-zeatin suppressed this activation. Thus, trans-zeatin played the role of the ZBPChl activator in chloroplast lysates from ten-day-old seedling and its inhibitor in lysates from 14-day-old seedlings. The combination of ZBPChl and trans-zeatin activated the RNA synthesis in lysates from lupine leaves, which indicates the absence of species-specificity in the ZBPChl action. It was shown that, in the heterologous transcription system, the principal factor of regulation was not a plant species, which was a source of the ZBPChl or chloroplast lysate, but the physiological state of plants used. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The Involvement of the Chloroplast Zeatin-Binding Protein in Hormone-Dependent Transcriptional Control of the Chloroplast Genome

Loading next page...
 
/lp/springer_journal/the-involvement-of-the-chloroplast-zeatin-binding-protein-in-hormone-Bg59mo3jU6
Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1013720513537
Publisher site
See Article on Publisher Site

Abstract

Using a multistep system for protein purification, including affinity chromatography on zeatin riboside–Sepharose, we isolated a zeatin-binding protein from the chloroplasts of the first leaf of ten-day-old barley seedlings (ZBPChl). In combination with trans-zeatin, this protein enhanced the RNA synthesis in the chloroplast lysate from the same leaves. A hormonal specificity of ZBPChl-induced transcriptional stimulation was demonstrated. The protein activated the chloroplast but not nuclear genome. Age-dependent changes in the ZBPChl functional activity and in the response of the chloroplast transcription system to this protein and trans-zeatin were observed. In the chloroplast lysate from aging first leaves of 14-day-old barley plants, ZBPChl from the leaves of 3-, 10-, and 14-day-old barley plants activated transcription in the absence of the hormone, and trans-zeatin suppressed this activation. Thus, trans-zeatin played the role of the ZBPChl activator in chloroplast lysates from ten-day-old seedling and its inhibitor in lysates from 14-day-old seedlings. The combination of ZBPChl and trans-zeatin activated the RNA synthesis in lysates from lupine leaves, which indicates the absence of species-specificity in the ZBPChl action. It was shown that, in the heterologous transcription system, the principal factor of regulation was not a plant species, which was a source of the ZBPChl or chloroplast lysate, but the physiological state of plants used.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off