The integrated approach to the investigation of soluble multisubunit protein complexes in cyanobacterial cells

The integrated approach to the investigation of soluble multisubunit protein complexes in... Functional proteomics is currently one of the most important tools used for the studying of complex intracellular processes involving a variety of proteins that interact with each other with different probability. Many of them, fulfilling their functions within the complex network of interactions, affect functions of other proteins. Despite the fact that many of the components of these “protein networks” could be previously described individually, it did not give complete details about nature and components of the proposed interactions under natural conditions. The article describes the methods (native gel electrophoresis, size exclusion chromatography, and immuneprecipitation), the most effective in the study of protein interactions and optimized for the study of soluble multisubunit protein complexes in the cells of photosynthetic bacteria using a unicellular cyanobacterium Synechococcus elongates PCC 7942. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

The integrated approach to the investigation of soluble multisubunit protein complexes in cyanobacterial cells

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Publisher
Springer US
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714010105
Publisher site
See Article on Publisher Site

Abstract

Functional proteomics is currently one of the most important tools used for the studying of complex intracellular processes involving a variety of proteins that interact with each other with different probability. Many of them, fulfilling their functions within the complex network of interactions, affect functions of other proteins. Despite the fact that many of the components of these “protein networks” could be previously described individually, it did not give complete details about nature and components of the proposed interactions under natural conditions. The article describes the methods (native gel electrophoresis, size exclusion chromatography, and immuneprecipitation), the most effective in the study of protein interactions and optimized for the study of soluble multisubunit protein complexes in the cells of photosynthetic bacteria using a unicellular cyanobacterium Synechococcus elongates PCC 7942.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Mar 7, 2014

References

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