ISSN 10227954, Russian Journal of Genetics, 2016, Vol. 52, No. 1, pp. 49–55. © Pleiades Publishing, Inc., 2016.
Original Russian Text © L.V. Obukhova, V.K. Shumny, 2016, published in Genetika, 2016, Vol. 52, No. 1, pp. 59–65.
The Institute of Cytology and Genetics of the Rus
sian Academy of Sciences has a huge collection of
aneuploid, substituted, nearly isogenic, and introgres
sive lines [1–4]. This collection is used for a number of
purposes , e.g., for the creation of chromosome
nomenclature . The collection contains a wide
range of samples created with the use of the Sara
tovskaya 29 common wheat cultivar (S29). This culti
var originates from old indigenous cultivars of the
Volga region ; it had a large cultivation area in Rus
sia in the last century. It has a number of useful traits:
high flour strength, high level of drought resistance,
high kernel percentage, and resistance to loose smut
and powdery mildew.
Another trait of the S29 cultivar is intravarietal
loci [6, 10, 11]; this
fact was not known when the collection was created.
Moreover, samples of the S29 cultivar heterozygous
gene alleles and cases of polymor
phism for the SSRmarker were found in the Republic
of Kazakhstan .
All of the various lines in the collection of Institute
of Cytology and Genetics obtained with the use of the
S29 cultivar  were created on the basis of linear
material originating from a ear of a single sample from
the Saratov State University in 1967. We described this
line of the S29 cultivar as isogenic for the products of
The original isogenic genetic material is valuable as
an important model for studying the influence of genes
on agronomical parameters. Moreover, the genetic
uniformity of samples ensures the correct interpreta
tion of results by any method of genetic analysis.
The S29 cultivar was derived from two common
wheat cultivars: Lutescens 55/11 (Lut55/11) is the
paternal line, and Albidum 24 (Al24) is the maternal
line. The paternal cultivar originated from locally bred
Poltavka (Pol) and Selivanovskiy Rusak (Rus) culti
vars. The maternal cultivar originated from Sarroza
(Sar), then the Pol cultivar, and the tetraploid Belo
turka (Bel) cultivar .
The purpose of this study was to prove the confor
mity of the S29 cultivar allelic composition with that
The Inheritance of Endosperm Storage Proteins by the Line
of the Saratovskaya 29 Cultivar of Common Wheat
from Its Parental Forms
L. V. Obukhova and V. K. Shumny
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia
Received January 20, 2015
—We ran a comparative analysis of storage proteins (gliadins, high (HMW) and lowmolecular
weight (LMW) glutenins, puroindolines, and exogenous pest alphaamylase inhibitors) in the Saratovskaya
29 cultivar line from the collection of a genetic engineering laboratory, its parental forms (Albidum 24 and
Lutescens 55/11), and distant ancestors (Poltavka, Selivanovskiy Rusak, Sarroza, and tetraploid Beloturka).
It was confirmed that the allelic states of storage proteins in the
loci originate from
ancestral forms from the collection of the Vavilov Institute of Plant Industry. Moreover, new alleles were
found in Lutescens 55/11 (
) and Selivanovskiy Rusak (
) cultivars from the collection of the
Institute of Cytology and Genetics. A new allelic state,
, was observed in the loci of the Poltavka cultivar
as a softgrain cultivar, and the
allele was found in the hardgrain Albidum 24 and Sarroza cultivars. It was
found that the expression rate of exogenous pest alphaamylase inhibitors in the Saratovskaya 29 cultivar line
is lower than that of ancestral cultivars (Albidum 24, Sarroza, Poltavka, and Beloturka). Such inhibitors are
absent in the paternal form Lutescens 55/11. A high expression rate of protein pest inhibitors for exogenous
amylases and puroindolines was observed in the Poltavka cultivar. The allelic composition of
newly studied in the Sarroza cultivar, which has some promising features. The Saratovskaya 29 cultivar line,
on the basis of which a wide range of diverse lines were created in the Institute of Cytology and Genetics, is
isogenic for all of the studied traits.
: common wheat, glutenins, gliadins, puroindolines, exogenous amylase inhibitors, Saratovskaya 29
cultivar line, ancestral forms, wheat