Arch Virol (1998) 143: 549–556
The inﬂuence of the milieu on the rate of neutralisation
of herpes simplex virus 1
C. E. Bolt
, J. A. Davies
, S. Randall
, and G. R. B. Skinner
Department of Infection, The Medical School, University of Birmingham,
Vaccine Research Trust, Birmingham, U.K.
Department of Virology, St. Bartholomew’s Hospital, London, U.K.
Accepted November 18, 1997
Summary. The rate of neutralisation of herpes simplex virus 1 was increased by
up to more than ﬁve hundred-fold when the virus suspension and antiserum were
each diluted to one hundred-fold in water instead of phosphate buffered saline.
This phenomenon, which was observed for two human positive sera and a rabbit
puriﬁed polyclonal antibody, may represent an unrecognised homeostatic mecha-
nism where neutralising antibody is ‘dilution-fast’ under physiological conditions
of transudation or pathological conditions of inﬂammation.
Kinetic neutralisation constants (k values) have been used for many years to quan-
tify neutralising antibody activity against a wide variety of viruses . k values
are calculated according to a formula which posits that the factorial reduction in
virus titre isproportional to antibody concentration and duration of virus-antibody
interaction under conditions of antibody excess:
where c = dilution of antiserum in test mixtue
t= time in minutes
= initial virus concentration
= virus concentration at time ‘t’