The immune modulatory effect of oregano (Origanum vulgare L.) essential oil on Tilapia zillii following intraperitoneal infection with Vibrio anguillarum

The immune modulatory effect of oregano (Origanum vulgare L.) essential oil on Tilapia zillii... The current study aimed to evaluate the possible effect of Origanum essential oil on innate immune parameters as well as the hematological profiles of Tilapia zillii following challenge with Vibrio anguillarum. Fifty-four of Tilapia zillii weighing 180 ± 10.2 g were randomly distributed into three identical closed recirculating seawater systems. The study included three groups (G1, G2, and G3) repeated in triplicates. Fish of the first two groups were fed on a basal diet without herbs, whereas fish of the last group were fed on a basal diet supplemented with Origanum essential oil at concentration 1 g kg−1 for 15 days. Subsequently, fish of G2 and G3 subjected to a peritoneal inflammation by intraperitoneally injecting V. anguillarum (5.5 × 105 CFU mL−1), whereas fish of G1 injected with saline and served as control. Fish of all groups were then sampled at 4, 12, and 24 h post injection. No mortalities were observed in both basal and Origanum fed groups. However, some specimens of fish fed basal diet showed dorsal fin erosions, eroded mouth, and detached skin. Although the kinetics of RBCs, WBCs, Hb, and differential leukocyte values remained unchanged in fish fed different diets at the beginning of the trial, significant increases in those values were observed in fish fed Origanum essential oil at 12 and 24 h post injection. Similarly, an augmentation of plasma proteases, antiproteases, and lysozyme activities were recorded in fish fed Origanum essential oil at the same particular sampling points. A significant enhancement in plasma bactericidal capacity was only recorded in fish fed Origanum essential oil at 12 and 24 h post challenge compared to those fed basal diet. In conclusion, Origanum essential oil had a pronounced influence on the innate immunity and increased the fish resistance to V. anguillarum. These data gave insight into the potential use of Origanum in prophylactic strategies against threatening pathogens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Aquaculture International Springer Journals

The immune modulatory effect of oregano (Origanum vulgare L.) essential oil on Tilapia zillii following intraperitoneal infection with Vibrio anguillarum

14 pages
Read Article

Loading next page...
 
/lp/springer_journal/the-immune-modulatory-effect-of-oregano-origanum-vulgare-l-essential-atH4Ci45Q0
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer International Publishing AG, part of Springer Nature
Subject
Life Sciences; Freshwater & Marine Ecology; Zoology
ISSN
0967-6120
eISSN
1573-143X
D.O.I.
10.1007/s10499-018-0274-y
Publisher site
See Article on Publisher Site

Abstract

The current study aimed to evaluate the possible effect of Origanum essential oil on innate immune parameters as well as the hematological profiles of Tilapia zillii following challenge with Vibrio anguillarum. Fifty-four of Tilapia zillii weighing 180 ± 10.2 g were randomly distributed into three identical closed recirculating seawater systems. The study included three groups (G1, G2, and G3) repeated in triplicates. Fish of the first two groups were fed on a basal diet without herbs, whereas fish of the last group were fed on a basal diet supplemented with Origanum essential oil at concentration 1 g kg−1 for 15 days. Subsequently, fish of G2 and G3 subjected to a peritoneal inflammation by intraperitoneally injecting V. anguillarum (5.5 × 105 CFU mL−1), whereas fish of G1 injected with saline and served as control. Fish of all groups were then sampled at 4, 12, and 24 h post injection. No mortalities were observed in both basal and Origanum fed groups. However, some specimens of fish fed basal diet showed dorsal fin erosions, eroded mouth, and detached skin. Although the kinetics of RBCs, WBCs, Hb, and differential leukocyte values remained unchanged in fish fed different diets at the beginning of the trial, significant increases in those values were observed in fish fed Origanum essential oil at 12 and 24 h post injection. Similarly, an augmentation of plasma proteases, antiproteases, and lysozyme activities were recorded in fish fed Origanum essential oil at the same particular sampling points. A significant enhancement in plasma bactericidal capacity was only recorded in fish fed Origanum essential oil at 12 and 24 h post challenge compared to those fed basal diet. In conclusion, Origanum essential oil had a pronounced influence on the innate immunity and increased the fish resistance to V. anguillarum. These data gave insight into the potential use of Origanum in prophylactic strategies against threatening pathogens.

Journal

Aquaculture International – Springer Journals

Published: May 29, 2018

References

  • Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi
    Adam, K; Sivropoulou, A; Kokkini, S; Lanaras, T; Arsenakis, M
  • Neutrophil and macrophage responses to inflammation in the peritoneal cavity of rainbow trout Oncorhynchus mykiss. A light and electron microscopic cytochemical study
    Afonso, A; Lousada, S; Silva, J; Ellis, AE; Silva, MT
  • Numerical taxonomy of Vibrionaceae isolated from cultured amberjack (Serioladumerili) and surrounding water
    Alcaide, E
  • Composition and antimicrobial activity of the essential oils of two Origanum spp
    Aligiannis, N; Kalpoutzakis, E; Mitaku, S; Chinou, IB
  • Pathological and immunohistochemical investigations in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) experimentally infected with Vibrio anguillarum
    Avci, H; Lu, SB; Cagirgan, H
  • Strain differences in non-specific immunity of tilapia Oreochromis niloticus following challenge with Vibrio parahaemolyticus
    Balfry, SK; Shariff, M; Iwama, GK
  • A calculated response: control of inflammation by the innate immune system
    Barton, GM
  • Bergey’s manual of determinative bacteriology
    Baumann, P; Furniss, AL
  • The acute phase response and innate immunity of fish
    Bayne, CJ; Gerwick, L
  • Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues
    Botsoglou, NA; Florou-Paneri, P; Christaki, E; Fletouris, DJ; Spais, AB
  • Effect of herbal extracts supplemented diets on non-specific immunity and resistance to Aeromonas hydrophila in Indian catfish (Mystus montanus)
    Chelladurai, G; Veni, T; Mohanraj, J; Nagarajan, R
  • Improving aquaculture production through better health and disease prevention the natural way
    Ching, CYY
  • Cellular and humoral immune responses of Senegalese sole, Solea senegalensis (Kaup), following challenge with two Photobacterium damselae subsp. piscicida strains from different geographical origins
    Costas, B; Rêgo, PCNP; Simões, I; Marques, JF; Castro-Cunha, M; Afonso, A
  • The effects of chronic exposure to the water soluble fraction of fuel oil on an Antarctic fish Pagothenia borchgkevinki
    Davison, W; Franklin, CE; Mckenzie, JC; Carey, PW
  • Studies on vibriosis among some marine fishes in Lake Temsah
    Eissa, IAM; Aly, SM; Derwa, HI; Fawzy, A
  • Antibacterial effect of some medicinal plant essential oils against bacterial fish pathogens
    Ekici, S; Diler, O; Didinen, BI; Kubilay, A
  • Immunity to bacteria in fish
    Ellis, AE
  • The use of immunostimulating herbs in fish. An overview of research
    Galina, J; Yin, G; Ardó, L; Jeney, Z
  • Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila
    Garcia, F; Pilarski, F; Onaka, EM; Moraes, FR; Martins, ML
  • Development of a PCR-based method for the detection of Listonella anguillarum in fish tissues and blood samples
    Gonzalez, SF; Osorio, CR; Santos, Y
  • Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds
    Gopalakannan, A; Arul, V
  • Basics in clinical nutrition: immune nutrition—nutrients which influence immunity: effect and mechanism of action
    Grimble, RF
  • Impact of plant products on innate and adaptive immune system of cultured finfish
    Harikrishnan, R; Balasundaram, C; Heo, MS
  • Effect of Inonotus obliquus enriched diet on hematology, immune response, and disease protection in kelp grouper, Epinephelus bruneus against Vibrio harveyi
    Harikrishnan, R; Balasundaram, C; Heo, MS
  • Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection
    Harikrishnan, R; Nisha Rani, M; Balasundaram, C
  • Characterization of the action of selected essential oil components on Gram-negative bacteria
    Helander, IM; Alakomi, HL; Latva-Kala, K; Mattila-Sandholm, T; Pol, I; Smid, EJ; Gorris, LGM; Wright, A
  • The genus Origanum (Labiatae) in Crete: distribution and essential oils
    Karousou, R; Kokkini, S
  • Insecticidal and genotoxic activities of oregano essential oils
    Karpouhtsis, I; Pardali, E; Feggou, E; Kokkini, S; Scouras, ZG; Mavragani-Tsipidou, P
  • Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food
    Kocić-Tanackov, SD; Dimić, GR; Tanackov, IJ; Pejin, DJ; Mojović, LV; Pejin, JD
  • Non-specific cellular responses of rainbow trout Vibrio anguillarum and its extracellular products
    Lamas, J; Santos, Y; Bruno, DW; Toranzo, AE; Anadon, R
  • A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol
    Lambert, RJW; Skandamis, PN; Coote, PJ; Nychas, GJE
  • Pathogenesis of gastroenteritis caused by Vibrio carchariae in cultured marine fish
    Lee, KK; Liu, PC; Chuang, WH
  • Amino acids and immune function
    Li, P; Yin, YL; Li, D; Kim, SW; Wu, GY
  • Dietary tryptophan and methionine as modulators of European seabass (Dicentrarchus labrax) immune status and inflammatory response
    Machado, M; Azeredo, R; Díaz-Rosales, P; Afonso, A; Peres, H; Oliva-Teles, A; Costas, B
  • Changes in plasma lysozyme and blood leucocyte levels of hatchery-reared Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta L.) during parr-smolt transformation
    Muona, M; Soivio, A
  • Antimicrobial mechanisms of fish phagocytes and their role in host defense
    Neumann, NF; Stafford, JL; Barreda, D; Ainsworth, AJ; Belosevic, M
  • Effect of dietary inclusion of Origanum vulgare extract on non-specific immune responses and hematological parameters of rainbow trout (Oncorhynchus mykiss)
    Pourmoghim, H; Haghighi, M; Rohani, MS
  • Thymol and carvacrol affect hybrid tilapia through the combination of direct stimulation and an intestinal microbiota-mediated effect: insights from a germ-free zebrafish model–3
    Ran, C; Hu, J; Liu, W; Liu, Z; He, S; Dan, BCT; Diem, NN; Ooi, EL; Zhou, Z
  • Assessment of synergistic efficacy of carvacrol and cymene against Edwardsiella tarda in vitro and in tilapia (Oreochromis niloticus)
    Rattanachaikunsopond, P; Phumklachor, P
  • Effect of dietary oregano (Origanium vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens
    Roofchaee, A; Irani, M; Ebrahimzadeh, MA; Akbari, MR
  • Changes in hydrolytic enzyme activities of naïve Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation
    Ross, NW; Firth, KJ; Wang, A; Burka, JF; Johnson, SC
  • Effect of Magnifera indica kernel as a feed additive on immunity and resistance to Aeromonas hydrophila in Labeo rohita fingerlings
    Sahu, S; Das, BK; Pradhan, J; Mohapatra, BC; Mishra, BK; Sarangi, N
  • Effect of Origanum vulgare as a feed additive on growth performance, feed utilization and whole body composition of Nile tilapia (Oreochromis niloticus) fingerlings challenged with pathogenic Aeromonas hydrophila
    Seden, MEA; Abbas, AE; Ahmed, MH
  • Antimicrobial and cytotoxic activities of Origanum essential oils
    Sivropoulou, A; Paranikolaou, E; Nikolaou, C; Kokkini, S; Lanaras, T; Arsenakis, M
  • Non-specific immune parameters of brood Indian major carp Labeo rohita and their seasonal variations
    Swain, P; Dash, S; Sahoo, PK; Routray, P; Sahoo, SK; Gupta, SD; Meher, PK
  • Medicinal plant derivatives as immunostimulants: an alternative to chemotherapeutics and antibiotics in aquaculture
    Vaseeharan, B; Thaya, R
  • Growth parameters, innate immune response and resistance to Listonella anguillarum of D. labrax fed carvacrol supplemented diets
    Volpatti, D; Chiara, B; Francesca, T; Marco, G
  • Effect of Sophora flavescens on non-specific immune response of tilapia (GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae
    Wu, YR; Gong, QF; Fang, H; Liang, WW; Chen, M; He, RJ
  • In vitro antibacterial activity of some medicinal plants oil against fish pathogens Lactococcus garvieae and Vibrio anguillarum
    Yildirim, P; Kubilay, A; Guney, S
  • Influence of carvacrol on the growth performance, hematological, non-specific immune and serum biochemistry parameters in rainbow trout (Oncorhynchus mykiss)
    Yilmaz, E; Ergün, S
  • Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus
    Yin, G; Jeney, G; Racz, T; Xu, P; Jun, X; Jeney, Z
  • Study on tetrazolium salt colorimetric assay for growth and survival of bacteria
    Zhang, J; Liu, X
  • Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus)
    Zheng, ZL; Tan, JYW; Liu, HY; Zhou, XH; Xiang, X; Wang, KY
  • In vivo neutralization of Cryptobia salmositica metallo-protease by α2-macroglobulin in the blood of rainbow trout Oncorhynchus mykiss and in brook charr Salvelinus fontinalis
    Zuo, X; Woo, PTK

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial