The herpes simplex virus type 1 ribonucleotide reductaseis required for acute retinal disease

The herpes simplex virus type 1 ribonucleotide reductaseis required for acute retinal disease We have used a herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR) null mutant (ICP6Δ) to determine if the HSV-1 RR is required for acute retinal disease. Injection of the ICP6Δ mutant into the vitreous induced mild transient signs of infection (vitreal infiltrate, retinal inflammation, and changes in retinal cytology). In contrast, the parental KOS and a revertant virus (ICP6Δ + 3.1) in which the RR gene had been restored, caused severe retinitis. Injection of media alone also induced mild transient signs of disease. Two months after infection, ICP6Δ injected eyes could not be distinguished from normal eyes. Repeated injection of ICP6Δ (3 times, 2 weeks apart) resulted in vitreal infiltrate near the site of injection but the retina did not appear damaged. The mutant, ICP6Δ, grew to peak titers to -fold lower and cleared faster than KOS or ICP6Δ + 3.1 in the injected eyes suggesting that the reduced virulence was due to reduced ability of the virus to grow. These results show that the viral RR is required for acute retinal disease. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

The herpes simplex virus type 1 ribonucleotide reductaseis required for acute retinal disease

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Publisher
Springer Journals
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050126
Publisher site
See Article on Publisher Site

Abstract

We have used a herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR) null mutant (ICP6Δ) to determine if the HSV-1 RR is required for acute retinal disease. Injection of the ICP6Δ mutant into the vitreous induced mild transient signs of infection (vitreal infiltrate, retinal inflammation, and changes in retinal cytology). In contrast, the parental KOS and a revertant virus (ICP6Δ + 3.1) in which the RR gene had been restored, caused severe retinitis. Injection of media alone also induced mild transient signs of disease. Two months after infection, ICP6Δ injected eyes could not be distinguished from normal eyes. Repeated injection of ICP6Δ (3 times, 2 weeks apart) resulted in vitreal infiltrate near the site of injection but the retina did not appear damaged. The mutant, ICP6Δ, grew to peak titers to -fold lower and cleared faster than KOS or ICP6Δ + 3.1 in the injected eyes suggesting that the reduced virulence was due to reduced ability of the virus to grow. These results show that the viral RR is required for acute retinal disease.

Journal

Archives of VirologySpringer Journals

Published: May 1, 1997

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